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Team:Paris/Modeling/f5
From 2008.igem.org
(Difference between revisions)
| Line 29: | Line 29: | ||
|value | |value | ||
|- | |- | ||
| - | |[expr( | + | |[expr(pFliL)] |
|expression rate of <br> pFliA '''with RBS E0032''' | |expression rate of <br> pFliA '''with RBS E0032''' | ||
|nM.s<sup>-1</sup> | |nM.s<sup>-1</sup> | ||
| Line 63: | Line 63: | ||
|value | |value | ||
|- | |- | ||
| - | |β<sub> | + | |β<sub>53</sub> |
| - | |production rate of FlhDC- | + | |production rate of FlhDC-pFliL '''with RBS E0032''' <br> β<sub>53</sub> |
|nM.s<sup>-1</sup> | |nM.s<sup>-1</sup> | ||
| | | | ||
|- | |- | ||
| - | |(K<sub> | + | |(K<sub>47</sub>/{coef<sub>flhDC</sub>}<sup>n<sub>47</sub></sup>) |
| - | |activation constant of FlhDC- | + | |activation constant of FlhDC-pFliL <br> K<sub>47</sub> |
| - | |nM<sup>n<sub> | + | |nM<sup>n<sub>47</sub></sup> |
| | | | ||
|- | |- | ||
| - | |n<sub> | + | |n<sub>47</sub> |
| - | |complexation order of FliA- | + | |complexation order of FliA-pFliL <br> n<sub>47</sub> |
|no dimension | |no dimension | ||
| | | | ||
|- | |- | ||
|- | |- | ||
| - | |β<sub> | + | |β<sub>54</sub> |
| - | |production rate of FliA- | + | |production rate of FliA-pFliL '''with RBS E0032''' <br> β<sub>54</sub> |
|nM.s<sup>-1</sup> | |nM.s<sup>-1</sup> | ||
| | | | ||
|- | |- | ||
| - | |(K<sub> | + | |(K<sub>48</sub>/{coef<sub>fliA</sub>}<sup>n<sub>48</sub></sup>) |
| - | |activation constant of OmpR-pFlhDC <br> K<sub> | + | |activation constant of OmpR-pFlhDC <br> K<sub>48</sub> |
| - | |nM<sup>n<sub> | + | |nM<sup>n<sub>48</sub></sup> |
| | | | ||
|- | |- | ||
| - | |n<sub> | + | |n<sub>48</sub> |
| - | |complexation order of OmpR-pFlhDC <br> n<sub> | + | |complexation order of OmpR-pFlhDC <br> n<sub>48</sub> |
|no dimension | |no dimension | ||
| | | | ||
Revision as of 16:06, 21 August 2008
and
so the expression
gives
and
and for calculated values of the TF,
and
| param | signification | unit | value |
| [expr(pFliL)] | expression rate of pFliA with RBS E0032 | nM.s-1 | see "findparam" need for 20 + 20 measures and 5x5 measures for the SUM? |
| γGFP | dilution-degradation rate of GFP(mut3b) | s-1 | ln(2)/3600 |
| [GFP] | GFP concentration at steady-state | nM | need for 20 + 20 measures and 5x5 measures for the SUM? |
| (fluorescence) | value of the observed fluorescence | au | need for 20 + 20 measures and 5x5 measures for the SUM? |
| conversion | conversion ration between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value |
| β53 | production rate of FlhDC-pFliL with RBS E0032 β53 | nM.s-1 | |
| (K47/{coefflhDC}n47) | activation constant of FlhDC-pFliL K47 | nMn47 | |
| n47 | complexation order of FliA-pFliL n47 | no dimension | |
| β54 | production rate of FliA-pFliL with RBS E0032 β54 | nM.s-1 | |
| (K48/{coeffliA}n48) | activation constant of OmpR-pFlhDC K48 | nMn48 | |
| n48 | complexation order of OmpR-pFlhDC n48 | no dimension |
Then, if we have time, we want to verify the expected relation
