Team:Paris/Modeling/f5

From 2008.igem.org

(Difference between revisions)
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[[Image:f5DCA.png|thumb]] At the steady-state, we have
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[[Image:f5DCA.png|thumb]]
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<center>[[Image:FlhDCeq.jpg]]</center>
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and
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<center>[[Image:FliAeq.jpg]]</center>
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so the expression
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<center>[[Image:f5expr.jpg]]</center>
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gives
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<center>[[Image:f5FlhDC.jpg]]</center>
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and
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<center>[[Image:f5FliA.jpg]]</center>
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and for ''calculated values of the TF'',
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<center>[[Image:f5FlhDCCalc.jpg]]</center>
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and
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<center>[[Image:f5FliACalc.jpg]]</center>
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<br><br>
<br><br>
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|''conversion''
|''conversion''
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|conversion ration between <br> fluorescence and concentration
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|conversion ratio between <br> fluorescence and concentration
|nM.au<sup>-1</sup>
|nM.au<sup>-1</sup>
|(1/79.429)
|(1/79.429)
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Then, if we have time, we want to verify the expected relation
Then, if we have time, we want to verify the expected relation
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<center>[[Image:SumpFliL.jpg]]</center>
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[[Image:SumpFliL.jpg|center]]

Revision as of 23:47, 29 September 2008

F5DCA.png



param signification unit value
[expr(pFliL)] expression rate of
pFliL with RBS E0032
nM.s-1 see "findparam"
need for 20 + 20 measures
and 5x5 measures for the SUM?
γGFP dilution-degradation rate
of GFP(mut3b)
min-1 0.0198
[GFP] GFP concentration at steady-state nM need for 20 + 20 measures
and 5x5 measures for the SUM?
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the SUM?
conversion conversion ratio between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value
β53 production rate of FlhDC-pFliL with RBS E0032
β53
nM.min-1
(K47/{coefflhDC}) activation constant of FlhDC-pFliL
K47
nM
n47 complexation order of FlhDC-pFliL
n47
no dimension
β54 production rate of FliA-pFliL with RBS E0032
β54
nM.min-1
(K48/{coeffliA}) activation constant of FliA-pFliL
K48
nM
n48 complexation order of FliA-pFliL
n48
no dimension



Then, if we have time, we want to verify the expected relation

SumpFliL.jpg