Team:Paris/Modeling/f5

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[[Image:f5DCA.png|thumb|[Team:Paris/Modeling/Protocol_Of_Characterisation|Characterisation Plasmid]]]
We have [FlhDC] = {coef<sub>flhDC</sub>}''expr(pTet)'' = {coef<sub>flhDC</sub>} &#131;1([aTc]<sub>i</sub>)
We have [FlhDC] = {coef<sub>flhDC</sub>}''expr(pTet)'' = {coef<sub>flhDC</sub>} &#131;1([aTc]<sub>i</sub>)

Revision as of 14:44, 26 October 2008

Characterisation Plasmid]

We have [FlhDC] = {coefflhDC}expr(pTet) = {coefflhDC} ƒ1([aTc]i)

and [FliA] = {coefFliA}expr(pBad) = {coefFliA} ƒ2([arab]i)

So, at steady-states,

F5.jpg



param signification unit value comments
[expr(pFlhDC)] expression rate of
pFlhDC with RBS E0032
nM.min-1 need for 20 mesures with well choosen values of [aTc]i
and for 20 mesures with well choosen values of [arab]i
and 5x5 measures for the relation below?
γGFP dilution-degradation rate
of GFP(mut3b)
min-1 0.0198
[GFP] GFP concentration at steady-state nM need for 20 + 20 measures
and 5x5 measures for the relation below?
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the relation below?
conversion conversion ratio between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value comments
β52 production rate of FlhDC-pFliL with RBS E0032
β52
nM.min-1
(K46/{coeffliA}) activation constant of FlhDC-pFliL
K46
nM
n46 complexation order of FlhDC-pFliL
n46
no dimension
β53 production rate of FliA-pFliL with RBS E0032
β53
nM.min-1
(K47/{coefomp}) activation constant of FliA-pFliL
K47
nM
n47 complexation order of FliA-pFliL
n47
no dimension



Then, if we have time, we want to verify the expected relation

SumpFliL.jpg