Team:Paris/Modeling/f5

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F5DCA.png
At the steady-state, we have
FlhDCeq.jpg

and

FliAeq.jpg

so the expression

F5expr.jpg

gives

F5FlhDC.jpg

and

F5FliA.jpg

and for calculated values of the TF,

F5FlhDCCalc.jpg

and

F5FliACalc.jpg



param signification unit value
[expr(pFliL)] expression rate of
pFliA with RBS E0032
nM.s-1 see "findparam"
need for 20 + 20 measures
and 5x5 measures for the SUM?
γGFP dilution-degradation rate
of GFP(mut3b)
s-1 ln(2)/3600
[GFP] GFP concentration at steady-state nM need for 20 + 20 measures
and 5x5 measures for the SUM?
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the SUM?
conversion conversion ration between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value
β53 production rate of FlhDC-pFliL with RBS E0032
β53
nM.s-1
(K47/{coefflhDC}n47) activation constant of FlhDC-pFliL
K47
nMn47
n47 complexation order of FliA-pFliL
n47
no dimension
β54 production rate of FliA-pFliL with RBS E0032
β54
nM.s-1
(K48/{coeffliA}n48) activation constant of OmpR-pFlhDC
K48
nMn48
n48 complexation order of OmpR-pFlhDC
n48
no dimension



Then, if we have time, we want to verify the expected relation

SumpFliL.jpg