Team:Paris/Modeling/f5

From 2008.igem.org

Revision as of 13:22, 6 September 2008 by Hugo (Talk | contribs)
F5DCA.png
At the steady-state, we have
FlhDCeq.jpg

and

FliAeq.jpg

so the expression

F5expr.jpg

gives

F5FlhDC.jpg

and

F5FliA.jpg

and for calculated values of the TF,

F5FlhDCCalc.jpg

and

F5FliACalc.jpg



param signification unit value
[expr(pFliL)] expression rate of
pFliL with RBS E0032
nM.s-1 see "findparam"
need for 20 + 20 measures
and 5x5 measures for the SUM?
γGFP dilution-degradation rate
of GFP(mut3b)
min-1 0.0198
[GFP] GFP concentration at steady-state nM need for 20 + 20 measures
and 5x5 measures for the SUM?
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the SUM?
conversion conversion ration between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value
β53 production rate of FlhDC-pFliL with RBS E0032
β53
nM.min-1
(K47/{coefflhDC}) activation constant of FlhDC-pFliL
K47
nM
n47 complexation order of FlhDC-pFliL
n47
no dimension
β54 production rate of FliA-pFliL with RBS E0032
β54
nM.min-1
(K48/{coeffliA}) activation constant of FliA-pFliL
K48
nM
n48 complexation order of FliA-pFliL
n48
no dimension



Then, if we have time, we want to verify the expected relation

SumpFliL.jpg