Team:Paris/Modeling/f7

From 2008.igem.org

Revision as of 18:43, 16 October 2008 by Hugo (Talk | contribs)
F7DCA.png

We have [FlhDC] = {coefflhDC}expr(pTet) = {coefflhDC} ƒ1([aTc]i)

and [FliA] = {coefFliA}expr(pBad) = {coefFliA} ƒ2([arab]i)

So, at steady-states,

F7a.jpg


param signification unit value comments
[expr(pFlhDC)] expression rate of
pFlhDC with RBS E0032
nM.min-1 need for 20 mesures with well choosen values of [aTc]i
and for 20 mesures with well choosen values of [arab]i
and 5x5 measures for the relation below?
γGFP dilution-degradation rate
of GFP(mut3b)
min-1 0.0198
[GFP] GFP concentration at steady-state nM need for 20 + 20 measures
and 5x5 measures for the relation below?
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the relation below?
conversion conversion ratio between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value comments
β13 production rate of FliA-pFlhDC with RBS E0032
β13
nM.min-1
(K12/{coeffliA}) activation constant of FliA-pFlhDC
K12
nM
n12 complexation order of FliA-pFlhDC
n12
no dimension
β2 production rate of OmpR-pFlhDC with RBS E0032
β2
nM.min-1
(K22/{coefomp}) activation constant of OmpR-pFlhDC
K22
nM
n22 complexation order of OmpR-pFlhDC
n22
no dimension



Then, if we have time, we want to verify the expected relation

SumpFlgB.jpg