Team:Paris/Modeling/f8

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F8DCA.png
At the steady-state, we have
FlhDCeq.jpg

and

FliAeq.jpg

so the expression

F8expr.jpg

gives

F8FlhDC.jpg

and

F8FliA.jpg

and for calculated values of the TF,

F8FlhDCCalc.jpg

and

F8FliACalc.jpg



param signification unit value
[expr(pFlhB)] expression rate of
pFlhB with RBS E0032
nM.s-1 see "findparam"
need for 20 + 20 measures
and 5x5 measures for the SUM?
γGFP dilution-degradation rate
of GFP(mut3b)
s-1 ln(2)/3600
[GFP] GFP concentration at steady-state nM need for 20 + 20 measures
and 5x5 measures for the SUM?
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the SUM?
conversion conversion ration between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value
β6 production rate of FlhDC-pFlhB with RBS E0032
β6
nM.s-1
(K4/{coefflhDC}n4) activation constant of FlhDC-pFlhB
K4
nMn4
n4 complexation order of FlhDC-pFlhB
n4
no dimension
β14 production rate of FliA-pFlhB with RBS E0032
β14
nM.s-1
(K10/{coeffliA}n10) activation constant of FliA-pFlhB
K10
nMn10
n10 complexation order of FliA-pFlhB
n10
no dimension



Then, if we have time, we want to verify the expected relation

SumpFlhB.jpg