Team:Paris/Modeling/f8

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F8DCA.png

We have [FlhDC] = {coefflhDC}expr(pTet) = {coefflhDC} ƒ1([aTc]i)

and [FliA] = {coefFliA}expr(pBad) = {coefFliA} ƒ2([arab]i)

So, at steady-states,

F8.jpg


param signification unit value comments
[expr(pFlhDC)] expression rate of
pFlhDC with RBS E0032
nM.min-1 need for 20 mesures with well choosen values of [aTc]i
and for 20 mesures with well choosen values of [arab]i
and 5x5 measures for the relation below?
γGFP dilution-degradation rate
of GFP(mut3b)
min-1 0.0198
[GFP] GFP concentration at steady-state nM need for 20 + 20 measures
and 5x5 measures for the relation below?
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the relation below?
conversion conversion ratio between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value comments
β6 production rate of FlhDC-pFlhB with RBS E0032
β6
nM.min-1
(K4/{coeffliA}) activation constant of FlhDC-pFlhB
K4
nM
n4 complexation order of FlhDC-pFlhB
n4
no dimension
β15 production rate of FliA-pFlhB with RBS E0032
β15
nM.min-1
(K11/{coefomp}) activation constant of FliA-pFlhB
K11
nM
n11 complexation order of FliA-pFlhB
n11
no dimension



Then, if we have time, we want to verify the expected relation

SumpFlhB.jpg