Team:Paris/Notebook/Freezer

From 2008.igem.org

(Difference between revisions)
(→Plasmids : MiniPrep Products)
(→Plasmids : MiniPrep Products)
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|PoPs to GFP converter (clones 1 + 2) [[Image:Part_icon_rbs.png |B0030]][[Image:Part_icon_reporter.png |B0030]][[Image:Part_icon_terminator.png |B0030]]
|PoPs to GFP converter (clones 1 + 2) [[Image:Part_icon_rbs.png |B0030]][[Image:Part_icon_reporter.png |B0030]][[Image:Part_icon_terminator.png |B0030]]
|MP7'.1
|MP7'.1
-
|
 
|[[Paris/July 18| July 18, 2007]]; [[Paris/July 24| July 24, 2007]]
|[[Paris/July 18| July 18, 2007]]; [[Paris/July 24| July 24, 2007]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
Line 252: Line 251:
|
|
| [[Paris/July 18| July 18]]
| [[Paris/July 18| July 18]]
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |MP9
 
-
|<bbpart>BBa_J61047</bbpart> in <bbpart>pSB1A2</bbpart>
 
-
|Cre ORF (clones 1 + 2) <partinfo>J61047 SpecifiedComponents</partinfo>
 
-
|MP9'.1, MP9'.2
 
-
| [[Paris/July 18| July 18]], [[Paris/July 24| July 24]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |MP10
 
-
|<bbpart>BBa_I0500</bbpart> in <bbpart>pSB2K3</bbpart>
 
-
|AraC pBAD (clones 1 + 2) <partinfo>I0500 SpecifiedComponents</partinfo>
 
-
|
 
-
| [[Paris/July 20| July 20]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |MP11
 
-
|<bbpart>BBa_pJ23107</bbpart> in <bbpart>p61002</bbpart>
 
-
|Medium constitutive promoter in J61002 (clones 3 + 4) <partinfo>pJ23107 SpecifiedComponents</partinfo>
 
-
|MP11'.3
 
-
| [[Paris/July 20| July 20]], [[Paris/July 23| July 23]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |MP12
 
-
|<bbpart>BBa_P1003</bbpart> in <bbpart>pSB1A2</bbpart>
 
-
|KanR <partinfo>P1003 SpecifiedComponents</partinfo>
 
-
|
 
-
| [[Paris/July 27| July 27]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |MP13
 
-
|<bbpart>BBa_J61002</bbpart>
 
-
|ptet-mRFP <partinfo>J61002 SpecifiedComponents</partinfo>
 
-
|MP13.1, MP13.2
 
-
| [[Paris/August 14| August 14]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |MP14
 
-
|<bbpart>BBa_J61025</bbpart>
 
-
|Medium constitutive promoter in J61002 (clones 1 + 2) <partinfo>BBa_J61025 SpecifiedComponents</partinfo>
 
-
|MP14.1, MP14.2
 
-
| [[Paris/August 14| August 14]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |L1
 
-
|<bbpart>BBa_I0500</bbpart> in <bbpart>J61002</bbpart>
 
-
|construct with pBad promotor regulating expression of mRFP <partinfo>I0500 SpecifiedComponents</partinfo>
 
-
|L1".1, L1".2
 
-
| [[Paris/August 14| August 14]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |
 
-
|AttB
 
-
|
 
-
|MP15
 
-
| [[Paris/August 14| August 14]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |
 
-
|AttP
 
-
|Finished
 
-
|MP16
 
-
| [[Paris/July 25| July 25]], [[Paris/July 27| July 27]], [[Paris/August 7| Aug 7]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |L2
 
-
|Lox66DapAE.coli in <bbpart>pSB1A2</bbpart>
 
-
|Insertion of lox66DapAE.coli construct as a biobrick in pSB1A2 plasmid
 
-
http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png
 
-
http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png
 
-
|
 
-
| [[Paris/July 25| July 25]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |L6
 
-
|<bbpart>BBa_B0030</bbpart>+<bbpart>BBa_J61047</bbpart>
 
-
|strong RBS(B0030)-Cre ORF
 
-
http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png
 
-
http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png
 
-
|L6.1, L6.2, L6.3, L6".1, L6".2
 
-
| [[Paris/July 25| July 25]], [[Paris/July 27| July 27]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |L9''
 
-
|lox66 in <bbpart>pSB1A2</bbpart>
 
-
|
 
-
|
 
-
|[[Paris/July 25| July 25]], [[Paris/July 30| July 30]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |L10''
 
-
|lox71 in <bbpart>pSB1A2</bbpart>
 
-
|
 
-
|
 
-
|[[Paris/July 25| July 25]], [[Paris/July 30| July 30]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |L12
 
-
|lox71-gfp in pSB1A2
 
-
|construction of the biobrick lox71-gfp
 
-
http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png
 
-
http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png
 
-
|L12.1 & L12.2
 
-
|[[Paris/July 30| July 30]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |L26
 
-
|araC/pbad>>RBS-Cre in <bbpart>J61002</bbpart>
 
-
|ligation ([[Paris/August 13| Aug.13]]) of RBS-Cre as a CDS behind pbad in <bbpart>J61002</bbpart>
 
-
|L26.1 (no more) & L26.2
 
-
|[[Paris/August 17| Aug. 17]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |L29
 
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|gfptripart-lox66 <bbpart>pSB1A2</bbpart>
 
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|ligation ([[Paris/August 13| Aug.13]]) of gfp-tripart in front of lox66
 
-
|L29.1 & L29.2
 
-
|[[Paris/August 17| Aug. 17]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |L30
 
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|dapAp>>mRFP in <bbpart>J61002</bbpart>
 
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|dapAp promotor placed([[Paris/August 13| Aug.13]]) upstream of mRFP <bbpart>J61002</bbpart>
 
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|L30A1 & L30A2
 
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|[[Paris/August 17| Aug. 17]]
 
-
|- style="background: #cccccc;"
 
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| style="background: #ccffcc;" |L31
 
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|lox71-<bbpart>B0015</bbpart>(T) in <bbpart>pSB1A2</bbpart>
 
-
|
 
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|L31.1 & L31.3
 
-
|[[Paris/August 20| Aug. 20]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |L32
 
-
|<bbpart>B0015</bbpart>(T)-lox66 in <bbpart>pSB1A2</bbpart>
 
-
|
 
-
|L32.1 & L32.2
 
-
|[[Paris/August 20| Aug. 20]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |L34
 
-
|pTet>>lox71-gfptripart in <bbpart>pSB1A2</bbpart>
 
-
|
 
-
|L34.1 & L34.2
 
-
|[[Paris/August 20| Aug. 20]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |L35
 
-
|araC/pBad>>lox71-gfptripart in <bbpart>pSB1A2</bbpart>
 
-
|il manque la seq. de 740-1410
 
-
|L35.1 & L35.2
 
-
|[[Paris/August 20| Aug. 20]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |L36
 
-
|lox66-mRFP
 
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|
 
-
|L36.1 & L36.2
 
-
|[[Paris/August 20| Aug. 20]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |L37
 
-
|lox66-RBS-dapAcoli
 
-
|
 
-
|L37.1 & L37.2
 
-
|[[Paris/August 20| Aug. 20]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |L39
 
-
|RBS-dapAcoli cloned in <bbpart>pSB1A2</bbpart>
 
-
|
 
-
|L39.1 & L39.2
 
-
|[[Paris/August 20| Aug. 20]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |L46
 
-
|pTet>>lox71-B0015(T) in <bbpart>pSB1A2</bbpart>
 
-
|
 
-
|L46.1
 
-
|[[Paris/September 18| Sep. 18]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |L53
 
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|B0015(T)-lox66-RBSDapAcoli in <bbpart>pSB1A2</bbpart>
 
-
|
 
-
|L53.7
 
-
|[[Paris/September 18| Sep. 18]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |L56
 
-
|B0015(T)-lox66-RBSdapAsubtilis in <bbpart>pSB1A2</bbpart>
 
-
|
 
-
|L56.3 (attention, seq a partiellement foiré, refaire seq)
 
-
|[[Paris/September 18| Sep. 18]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |L59
 
-
|pTet>>lox71-gfptripart-lox66-mRFP <bbpart>pSB1A2</bbpart>
 
-
|
 
-
|L59.6 (attention, seq avec O40 a foiré, mais les extrémités sont bonnes, je pense que c ok car les colonies sont vertes)
 
-
|[[Paris/September 18| Sep. 18]]
 
-
|- style="background: #cccccc;"
 
-
| style="background: #ccffcc;" |L64
 
-
|RBSdapAsubtilis cloned in <bbpart>pSB1A2</bbpart>
 
-
|
 
-
|L564.1 & L64.2
 
-
|[[Paris/September 18| Sep. 18]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |MPT1
 
-
|Lox66-DapAColi
 
-
|pTOPO P5
 
-
|MPT1.1, MPT1.2
 
-
|[[Paris/August 7| Aug 7]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |MPT3
 
-
|Lox66-DapASubtilis
 
-
|pTOPO P6
 
-
|MPT3.2, MPT3.1
 
-
|[[Paris/August 7| Aug 7]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |MPT4
 
-
|RBS-DapAColi
 
-
|pTOPO P8
 
-
|MPT4.1, .2, .3, .4& .5
 
-
|[[Paris/August 7| Aug 7]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |MPT5
 
-
|RBS-DapASubtilis
 
-
|pTOPO P9
 
-
|MPT5.1, .2 &.3
 
-
|[[Paris/August 7| Aug 7]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |MP15
 
-
|pKD46
 
-
|
 
-
|
 
-
|[[Paris/October 2| Oct 2]]
 
|}
|}

Revision as of 12:38, 22 July 2008

PCR Products

PCR products
Number Name Oligo F Oligo R Matrix Length Comments Product Left ? []ng/µL Date
P1 Lox71-FtsA-FtsZ-1 O3 Lox71-FtsA-F O4 DEcoR1-FtsZ-R MG1655 1537 Ø
P2 FtsZ-2 O5 DEcoR1-FtsZ-F O2 FtsZ-R MG1655 1088 Ø
P3 DGAT-1 27 ForDGAT1 13 DPst1-DGAT-R Miniprep pKS::DGAT 1087 July 31
P4 DGAT-2 12 DPst1-DGAT 27 Rev-DGAT1 Miniprep pKS::DGAT 396 July 31
P5 Lox66-DapAColi O6 Lox66-DapAColi-F O7 DapAColi-R MG1655 985 Ø July 17, July 30
P6 Lox66-DapASubtilis O8 Lox66-DapASubt-F O9 DapASubt-R B.Subtilis 168 979 July 17, July 27, July 30
P7 Lox71-FtsZ O1 Lox71-FtsZ-F O2 FtsZ-R MG1655 1260 EcoRI site in FtsZ not mutated July 17, July 30
P8 RBS-DapAColi O20 RBS-DapAColi-F O7 DapAColi-R MG1655 952 B0030-DapAColi July 23, July 30
P9 RBS-DapASubtilis O21 RBS-DapASubt-F O9 DapASubt-R B.Subtilis 168 946 B0030-DapASubtilis July 23, July 27
P10 Lox71-FtsA-FtsZ O3 Lox71-FtsA-F O2 FtsZ-R MG1655 2580 EcoRI site in FtsZ not mutated July 23
P11 Lox71-KmR-Lox66 O10 Lox71-KmR-F O11 Lox66-KmR-R MP12 1084 July 27, July 30
P13 DGAT deltaPst-1 ForDGAT1 Megaprimer DGAT2 DNA plasmid (pKS::DGAT): 1443 August 1
P14 DGAT deltaPst-1 biobrick ForDGAT1bb RevDGAT1bb DGAT deltaPst1 1504 August 2 Oct. 18
P15 PacI [pTet>>lox-GFP-loxmRFP] XmaI O47 O48 [pTet>>lox-GFP-loxmRFP] L59.6 2kb August 2
P16 PrefixBB-frt1-CmR-frt2-SufixBB O51 O52 pY-2C-IntC 1kb Oct. 15
P17 PrefixBB-frt1-CmR-frt2-T1T2-SufixBB O51 O53 pY-2C-IntC >1kb Oct. 18

Plasmids : MiniPrep Products

Plasmids: MiniPrep Products
Number Name Description Products in use Date
MP1 pJ23100 in J61002 Strong constitutive promoter in J61002(clones 1 + 2) pJ23100 July 15, 2007; Aug. 17, 2007
MP2 pJ23107 in J61002 Medium constitutive promoter in J61002 (clones 1 + 2) pJ23100 MP2.2 July 15, 2007; Aug. 17, 2007
MP3 B0015 in pSB1AK3 Double terminator (clones 1 + 2) B0015B0015 MP3.3, MP3.4 August 14, 2007
MP4 I0500 in pSB2K3 AraC pBAD (clones 1 + 2) pJ23100 July 16, 2007
MP5 B0030 in pSB1A2 Strong RBS (clones 1 + 2) B0030 July 18, 2007
MP6 E0422 in pSB1A2 ECFP (RBS+LVA+Term) (clones 1 + 2) B0030B0030B0030B0030 July 18, 2007; July 24, 2007
MP7 E0241 in pSB1A2 PoPs to GFP converter (clones 1 + 2) B0030B0030B0030 MP7'.1 July 18, 2007; July 24, 2007
MP8 <bbpart>BBa_E0840</bbpart> in <bbpart>pSB1A2</bbpart> gfp tri-part; strong rbs (clones 1 + 2) <partinfo>E0840 SpecifiedComponents</partinfo> July 18
Retrieved from "http://2008.igem.org/Team:Paris/Notebook/Freezer"