Team:Paris/Notebook/Freezer

From 2008.igem.org

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== PCR products ==
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{|
|- style="background: #ccccff;"
|- style="background: #ccccff;"
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! colspan="5" style="background: #ccccff;" | PCR products
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! colspan="10" style="background: #ccccff;" | PCR products
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|width=5%| Number
|width=5%| Number
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|width=5%| Date
|width=5%| Date
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
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| style="background: #ccffcc;" |P1
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| style="background: #ccffcc;" |P3
-
|  
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|DGAT-1
-
|  
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|27 ForDGAT1
-
|  
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|13 DPst1-DGAT-R
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|  
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|Miniprep pKS::DGAT
-
|  
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|1087
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||>1kb
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|[[Paris/October 18| Oct. 18]]
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|[[Paris/July_31|July 31]]
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|}
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== Plasmids : MiniPrep Products==
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{|
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|- style="background: #ccccff;"
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! colspan="5" style="background: #ccccff;" | Plasmids: MiniPrep Products
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|- style="background: #ccccff; text-align: center;"
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|width=5%| Number
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|width=5%| Name
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|width=50%| Description
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|width=15%| Products in use
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|width=25%| Date
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|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
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| style="background: #ccffcc;" |MP1
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| style="background: #ccffcc;" |P4
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|[http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002]
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|DGAT-2
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|Strong constitutive promoter in J61002(clones 1 + 2) [[Image:Part_icon_regulatory.png|pJ23100]]
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|12 DPst1-DGAT
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|27 Rev-DGAT1
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|Miniprep pKS::DGAT
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|396
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|
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|[[Paris/July 15| July 15, 2007]]; [[Paris/August 17| Aug. 17, 2007]]
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |MP2
 
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|[http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002]
 
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|Medium constitutive promoter in J61002 (clones 1 + 2) [[Image:Part_icon_regulatory.png|pJ23100]]
 
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|MP2.2
 
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| [[Paris/July 15| July 15, 2007]]; [[Paris/August 17| Aug. 17, 2007]]
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |MP3
 
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|[http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1Ak3 pSB1AK3]
 
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|Double terminator (clones 1 + 2) [[Image:Part_icon_terminator.png| B0015]][[Image:Part_icon_terminator.png| B0015]]
 
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|MP3.3, MP3.4
 
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| [[Paris/August 14 2007| August 14, 2007]]
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |MP4
 
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|[http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3]
 
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|AraC pBAD (clones 1 + 2) [[Image:Part_icon_regulatory.png | pJ23100]]
 
|
|
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| [[Paris/July 16| July 16, 2007]]
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |MP5
 
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|[http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]
 
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|Strong RBS (clones 1 + 2) [[Image:Part_icon_rbs.png |B0030]]
 
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|
 
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| [[Paris/July 18| July 18, 2007]]
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |MP6
 
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|[http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]
 
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|ECFP (RBS+LVA+Term) (clones 1 + 2) [[Image:Part_icon_rbs.png |B0030]][[Image:Part_icon_reporter.png |B0030]][[Image:Part_icon_terminator.png |B0030]][[Image:Part_icon_terminator.png |B0030]]
 
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|
 
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| [[Paris/July 18| July 18, 2007]]; [[Paris/July 24| July 24, 2007]]
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |MP7
 
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|[http://partsregistry.org/Part:BBa_E0241 E0241] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2]
 
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|PoPs to GFP converter (clones 1 + 2) [[Image:Part_icon_rbs.png |B0030]][[Image:Part_icon_reporter.png |B0030]][[Image:Part_icon_terminator.png |B0030]]
 
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|MP7'.1
 
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|[[Paris/July 18| July 18, 2007]]; [[Paris/July 24| July 24, 2007]]
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |MP8
 
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|<bbpart>BBa_E0840</bbpart> in <bbpart>pSB1A2</bbpart>
 
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|gfp tri-part; strong rbs (clones 1 + 2) <partinfo>E0840 SpecifiedComponents</partinfo>
 
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|
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| [[Paris/July 18| July 18]]
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|[[Paris/July_31|July 31]]
|}
|}

Revision as of 12:51, 22 July 2008

PCR products

PCR products
Number Name Oligo F Oligo R Matrix Length Comments Product Left ? []ng/µL Date
P3 DGAT-1 27 ForDGAT1 13 DPst1-DGAT-R Miniprep pKS::DGAT 1087 July 31
P4 DGAT-2 12 DPst1-DGAT 27 Rev-DGAT1 Miniprep pKS::DGAT 396 July 31
Retrieved from "http://2008.igem.org/Team:Paris/Notebook/Freezer"