"
Team:Paris/Notebook/Freezer
From 2008.igem.org
(Difference between revisions)
(→Plasmids: MiniPrep Products) |
(→Plasmids: MiniPrep Products) |
||
| Line 53: | Line 53: | ||
|width=20| Date | |width=20| Date | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc; | + | | style="background: #ccffcc;" |MP1 |
|align="center"|[http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002] | |align="center"|[http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002] | ||
|align="center"|Strong constitutive promoter in J61002 (clones 1 + 2) [[Image:Part_icon_regulatory.png]] | |align="center"|Strong constitutive promoter in J61002 (clones 1 + 2) [[Image:Part_icon_regulatory.png]] | ||
| Line 59: | Line 59: | ||
| [[Paris/July 15| July 15, 2007]]; [[Paris/August 17| Aug. 17, 2007]] | | [[Paris/July 15| July 15, 2007]]; [[Paris/August 17| Aug. 17, 2007]] | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc; | + | | style="background: #ccffcc;" |MP2 |
|align="center"|[http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002] | |align="center"|[http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002] | ||
|align="center"|Medium constitutive promoter in J61002 (clones 1 + 2) [[Image:Part_icon_regulatory.png]] | |align="center"|Medium constitutive promoter in J61002 (clones 1 + 2) [[Image:Part_icon_regulatory.png]] | ||
| Line 65: | Line 65: | ||
|align="center"| [[Paris/July 15| July 15, 2007]]; [[Paris/August 17| Aug. 17, 2007]] | |align="center"| [[Paris/July 15| July 15, 2007]]; [[Paris/August 17| Aug. 17, 2007]] | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc; | + | | style="background: #ccffcc;" |MP3 |
|align="center"|[http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1AK3 pSB1AK3] | |align="center"|[http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1AK3 pSB1AK3] | ||
|align="center"|Double terminator (clones 1 + 2) [[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]] | |align="center"|Double terminator (clones 1 + 2) [[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]] | ||
| Line 71: | Line 71: | ||
|align="center"| [[Paris/August 14| August 14, 2007]] | |align="center"| [[Paris/August 14| August 14, 2007]] | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc; | + | | style="background: #ccffcc;" |MP4 |
|align="center"|[http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] | |align="center"|[http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] | ||
|align="center"|AraC pBAD (clones 1 + 2) [[Image:Part_icon_regulatory.png]] | |align="center"|AraC pBAD (clones 1 + 2) [[Image:Part_icon_regulatory.png]] | ||
| Line 77: | Line 77: | ||
|align="center"| [[Paris/July 16| July 16, 2007]] | |align="center"| [[Paris/July 16| July 16, 2007]] | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc; | + | | style="background: #ccffcc;" |MP5 |
|align="center"|[http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] | |align="center"|[http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] | ||
|align="center"|Strong RBS (clones 1 + 2) [[Image:Part_icon_rbs.png]] | |align="center"|Strong RBS (clones 1 + 2) [[Image:Part_icon_rbs.png]] | ||
| Line 83: | Line 83: | ||
|align="center"| [[Paris/July 18| July 18, 2007]] | |align="center"| [[Paris/July 18| July 18, 2007]] | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc; | + | | style="background: #ccffcc;" |MP6 |
|align="center"|[http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] | |align="center"|[http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] | ||
|align="center"|ECFP (RBS+LVA+Term) (clones 1 + 2) [[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]|align="center"[[Image:Part_icon_terminator.png]] | |align="center"|ECFP (RBS+LVA+Term) (clones 1 + 2) [[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]|align="center"[[Image:Part_icon_terminator.png]] | ||
| Line 89: | Line 89: | ||
|align="center"| [[Paris/July 18| July 18, 2007]]; [[Paris/July 24| July 24, 2007]] | |align="center"| [[Paris/July 18| July 18, 2007]]; [[Paris/July 24| July 24, 2007]] | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc; | + | | style="background: #ccffcc;" |MP8 |
|align="center"|[http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] | |align="center"|[http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] | ||
| - | |align="center"|gfp tri-part; strong rbs (clones 1 + 2) [[Image:Part_icon_rbs.png]]| | + | |align="center"|gfp tri-part; strong rbs (clones 1 + 2) [[Image:Part_icon_rbs.png]]|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]] |
| | | | ||
|align="center"| [[Paris/July 18| July 18, 2007]] | |align="center"| [[Paris/July 18| July 18, 2007]] | ||
|} | |} | ||
Revision as of 15:25, 22 July 2008
PCR products
| PCR products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Name | Oligo F | Oligo R | Matrix | Length | Comments | Product Left ? | []ng/µL | Date |
| [[ ]] | |||||||||
| [[ ]] | |||||||||
Plasmids: MiniPrep Products
| Plasmids: MiniPrep Products | ||||
|---|---|---|---|---|
| Number | Name | Description | Products in use | Date |
| MP1 | pJ23100 in J61002 | Strong constitutive promoter in J61002 (clones 1 + 2) 
| July 15, 2007; Aug. 17, 2007 | |
| MP2 | pJ23107 in J61002 | Medium constitutive promoter in J61002 (clones 1 + 2)
| MP2.2 | July 15, 2007; Aug. 17, 2007 |
| MP3 | B0015 in pSB1AK3 | Double terminator (clones 1 + 2) 
| MP3.3, MP3.4 | August 14, 2007 |
| MP4 | I0500 in pSB2K3 | AraC pBAD (clones 1 + 2)
| July 16, 2007 | |
| MP5 | B0030 in pSB1A2 | Strong RBS (clones 1 + 2) 
| July 18, 2007 | |
| MP6 | E0422 in pSB1A2 | ECFP (RBS+LVA+Term) (clones 1 + 2)  |align="center"
| MP6'.1, MP6'.2 | July 18, 2007; July 24, 2007 |
| MP8 | E0840 in pSB1A2 | gfp tri-part; strong rbs (clones 1 + 2) |
| July 18, 2007 | |
