Team:Paris/Notebook/Freezer
From 2008.igem.org
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|align="center"|[http://partsregistry.org/Part:BBa_J23101 J23101] | |align="center"|[http://partsregistry.org/Part:BBa_J23101 J23101] | ||
|align="center"|[[Image:Part_icon_regulatory.png]] | |align="center"|[[Image:Part_icon_regulatory.png]] | ||
- | |align="center"| | + | |align="center"|Strongest Constitutive promoter |
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|align="center"|2008 | |align="center"|2008 | ||
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|align="center"|[http://partsregistry.org/Part:BBa_J23109 J23109] | |align="center"|[http://partsregistry.org/Part:BBa_J23109 J23109] | ||
|align="center"|[[Image:Part_icon_regulatory.png]] | |align="center"|[[Image:Part_icon_regulatory.png]] | ||
- | |align="center"| | + | |align="center"|Weak Constitutive promoter |
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|align="center"|2008 | |align="center"|2008 |
Revision as of 18:02, 22 July 2008
PCR products
PCR products | |||||||||
---|---|---|---|---|---|---|---|---|---|
Number | Name | Oligo F | Oligo R | Matrix | Length | Comments | Product Left ? | []ng/µL | Date |
[[ ]] | |||||||||
[[ ]] |
Plasmids: MiniPrep Products
Plasmids: MiniPrep Products | |||||
---|---|---|---|---|---|
Number | Name | Biobricks | Description | Products in use | Date |
MP1 | pJ23100 in J61002 | Strong constitutive promoter in J61002 (clones 1 + 2) | 2007 | ||
MP2 | pJ23107 in J61002 | Medium constitutive promoter in J61002 (clones 1 + 2) | MP2.2 | 2007 | |
MP3 | B0015 in pSB1AK3 | Double terminator (clones 1 + 2) | MP3.3, MP3.4 | 2007 | |
MP4 | I0500 in pSB2K3 | AraC pBAD (clones 1 + 2) | 2007 | ||
MP5 | B0030 in pSB1A2 | Strong RBS (clones 1 + 2) | 2007 | ||
MP6 | E0422 in pSB1A2 | ECFP (RBS+LVA+Term) (clones 1 + 2) | MP6'.1, MP6'.2 | 2007 | |
MP8 | E0840 in pSB1A2 | gfp tri-part; strong rbs (clones 1 + 2) | 2007 |
Plasmids: MiniPrep "in preparation" | |||||
---|---|---|---|---|---|
Number | Name | Biobricks | Description | Products in use | Date |
B0034 | Strongest RBS | 2008 | |||
C0040 | tetR repressor | 2008 | |||
C0079 | lasR activator with LVA Tag | 2008 | |||
C0179 | lasR activator | 2007 | |||
E0030 | YFP | 2008 | |||
E0040 | GFP | 2008 | |||
E1010 | mRFP | 2008 | |||
J23101 | Strongest Constitutive promoter | 2008 | |||
J23109 | Weak Constitutive promoter | 2008 | |||
R0040 | tetR repressible promoter | 2008 | |||
R0079 | promoter (lasr & PAI regulated) | 2008 | |||
S03154 | intermediate construction | 2008 | |||
S03879 | intermediate construction | 2008 |
Digestion Products
PURPLE = Vector
GREEN = Insert
Digestion Products | |||||||||
---|---|---|---|---|---|---|---|---|---|
Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description | Product Left | []ng/µL | Date |
D1 | <bbpart>pJ23100</bbpart> | <bbpart>pJ23100</bbpart> | Spe1 | Pst1 | 2096 | Vector with the J23100 promoter ready for insertion of a RBS/CDS insert | Ø (24/09/07) | 34 | July 17 |
D2 | <bbpart>mRFP dblTer</bbpart> | <bbpart>pJ23100</bbpart> | Spe1 | Pst1 | 887 | 27 | July 17 |