Team:Paris/Notebook/Freezer

From 2008.igem.org

(Difference between revisions)
(→Plasmids: MiniPrep Products)
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== Plasmids: MiniPrep Products ==
== Plasmids: MiniPrep Products ==
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{|
{|
|- style="background: #ccccff;"
|- style="background: #ccccff;"
-
! colspan="6" style="background: #ccccff;" | Plasmids: MiniPrep in production
+
! colspan="6" style="background: #ccccff;" | Plasmids: MiniPrep "in preparation"
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|width=5%| Number
|width=5%| Number
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|align="center"|2008
|align="center"|2008
|}
|}
 +
 +
== Digestion Products ==
== Digestion Products ==
Line 249: Line 253:
|27
|27
|[[Paris/July 17| July 17]]
|[[Paris/July 17| July 17]]
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;" |D3
 
-
|<bbpart>pJ23107</bbpart>
 
-
|<bbpart>pJ23107</bbpart>
 
-
|Spe1
 
-
|Pst1
 
-
|2096
 
-
|Vector with the J23107 promoter ready for insertion of a RBS/CDS insert
 
-
|Ø (24/09/07)
 
-
|32
 
-
|[[Paris/July 17| July 17]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;" |D4
 
-
|<bbpart>mRFP dblTer</bbpart>
 
-
|<bbpart>pJ23107</bbpart>
 
-
|Spe1
 
-
|Pst1
 
-
|887
 
-
|
 
-
|
 
-
|25
 
-
|[[Paris/July 17| July 17]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;" |D5
 
-
|<bbpart>pJ23100</bbpart>
 
-
|<bbpart>pJ23100</bbpart>
 
-
|EcoR1
 
-
|Spe1
 
-
|2925
 
-
|The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
 
-
|
 
-
|28&29
 
-
|[[Paris/July 17| July 17]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;" |D6
 
-
|<bbpart>pJ23107</bbpart>
 
-
|<bbpart>pJ23107</bbpart>
 
-
|EcoR1
 
-
|Spe1
 
-
|2925
 
-
|The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
 
-
|
 
-
|31&27
 
-
|[[Paris/July 17| July 17]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D7
 
-
|Lox66 DapA E.coli
 
-
|Lox66 DapA E.coli (PCR product)
 
-
|EcoR1
 
-
|Pst1
 
-
|977
 
-
|
 
-
|
 
-
|24
 
-
|[[Paris/July 17| July 17]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D8
 
-
|Lox66 DapA E.coli
 
-
|Lox66 DapA E.coli (PCR product)
 
-
|Xba1
 
-
|Pst1
 
-
|962
 
-
|
 
-
|
 
-
|44
 
-
|[[Paris/July 17| July 17]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;" |D9
 
-
|<bbpart>pJ23100</bbpart> ES vector
 
-
|<bbpart>pJ23100</bbpart> (MP1.1 & MP1.2)
 
-
|EcoI
 
-
|SpeI
 
-
|2925
 
-
|Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert)
 
-
|
 
-
|80&43
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D10
 
-
|<bbpart>pJ23100</bbpart> promoter fw-insert
 
-
|<bbpart>pJ23100</bbpart> (MP1.1 & MP1.2)
 
-
|EcoI
 
-
|SpeI
 
-
|58
 
-
|J23100 promoter ready for use as a fw-insert
 
-
|
 
-
|35&28
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;" |D11
 
-
|<bbpart>pJ23107</bbpart> ES vector
 
-
|<bbpart>pJ23107</bbpart> (MP2.1, MP2.2, MP2.3 & MP2.4)
 
-
|EcoRI
 
-
|SpeI
 
-
|2925
 
-
|Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter
 
-
|
 
-
|49,35,28&41
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D12
 
-
|<bbpart>pJ23107</bbpart> promoter fw-insert
 
-
|<bbpart>pJ23107</bbpart> (MP2.1, MP2.2, MP2.3 & MP2.4)
 
-
|EcoRI
 
-
|SpeI
 
-
|58
 
-
|J23107 promoter ready for usage as a fw-insert
 
-
|
 
-
|23,25,32&32
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D13
 
-
|<bbpart>mRFP</bbpart>
 
-
|<bbpart>pJ23107</bbpart> (MP2.2, MP2.3 & MP2.4)
 
-
|SpeI
 
-
|PstI
 
-
|887
 
-
|mRFP RBS/CDS/T sequence, can it be used as a backward insert? => yes but you will not be able to add any biobrick after it
 
-
|
 
-
|21&41
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D14
 
-
|Cre ORF
 
-
|<bbpart>J61047</bbpart> MP9.1 & MP9.2
 
-
|XbaI
 
-
|PstI
 
-
|1063
 
-
|Cre ORF extracted for usage as a bw-insert
 
-
|Ø D14.1
 
-
|38&41
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;" |D15
 
-
|<bbpart>B0030</bbpart> bw-vector
 
-
|<bbpart>B0030</bbpart> MP5.1 & MP5.2
 
-
|SpeI
 
-
|PstI
 
-
|2076
 
-
|bacward vector for cloning a CDS bw-insert after the B0030 RBS
 
-
|
 
-
|87&42
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D18
 
-
|BB dig. lox66DapAE.coli
 
-
|lox66-DapAE.coli P5 PCR product
 
-
|EcoRI
 
-
|PstI
 
-
|~960
 
-
|PCR product digested for cloning as a BioBrick
 
-
|Ø
 
-
|44
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D19
 
-
|BB dig. lox66DapAsubtilis
 
-
|lox66-DapAsubtilis P6 PCR product
 
-
|EcoRI
 
-
|PstI
 
-
|~960
 
-
|PCR product digested for cloning as a BioBrick
 
-
|Ø
 
-
|
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D19'
 
-
|BB dig. lox66DapAsubtilis
 
-
|lox66-DapAsubtilis P6' PCR product
 
-
|EcoRI
 
-
|PstI
 
-
|~960
 
-
|PCR product digested for cloning as a BioBrick
 
-
|
 
-
|16
 
-
|[[Paris/July 30| July 30]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D20
 
-
|bw-insert lox66-DapAE.coli
 
-
|lox66-DapAE.coli P5 PCR product
 
-
|XbaI
 
-
|PstI
 
-
|~960
 
-
|PCR product digested for usage as a backward insert
 
-
|Ø
 
-
|
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D21
 
-
|bw-insert lox66-DapAsubtilis
 
-
|lox66-DapAsubtilis P6 PCR product
 
-
|XbaI
 
-
|PstI
 
-
|~960
 
-
|PCR product digested for usage as a backward insert
 
-
|Ø
 
-
|
 
-
|[[Paris/July 21| July 21]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D21'
 
-
|bw-insert lox66-DapAsubtilis
 
-
|lox66-DapAsubtilis P6' PCR product
 
-
|XbaI
 
-
|PstI
 
-
|~960
 
-
|PCR product digested for cloning as a BI
 
-
|
 
-
|34
 
-
|[[Paris/July 30| July 30]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;" |D22
 
-
|pSB1A2 open vector
 
-
|<bbpart>BBa_J61047</bbpart> (MP9.1 & MP9.2)
 
-
|EcoI
 
-
|PstI
 
-
|Ø D22.1
 
-
|Open pSB1A2 vector for BioBrick EcoRI/PstI insertio
 
-
|Ø (24/09/07)
 
-
|D22.2:59
 
-
|[[Paris/July 23| July 23]],[[Paris/August 13 | Aug. 13 ]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D23
 
-
|AraC/pBad promoter fw-insert
 
-
|<bbpart>BBa_I0500</bbpart> (MP4.1 & MP4.2)
 
-
|EcoI
 
-
|SpeI
 
-
|1233
 
-
|AraC/pBad promoter ready for use as a forward insert
 
-
|Ø D23.1
 
-
|93&53
 
-
|[[Paris/July 23| July 23]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D24
 
-
|eCFP bw-insert
 
-
|<bbpart>BBa_E0422</bbpart> (MP6.1 & MP6.2)
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|eCFP (RBS-OFR_LVA-T) ready for use as a backward insert
 
-
|
 
-
|56&55
 
-
|[[Paris/July 23| July 23]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D26
 
-
|gfp-tripart
 
-
|<bbpart>BBa_E0840</bbpart> (MP8.1 & MP8.2)
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert
 
-
|
 
-
|47&47
 
-
|[[Paris/July 23| July 23]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D27
 
-
|lox71-ftsZ bw-insert
 
-
|lox71-ftsZ PCR product P7
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|lox71-ftsZ(unmutated) ready for use as a backward insert
 
-
|Ø
 
-
|
 
-
|[[Paris/July 23| July 23]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D27'
 
-
|lox71-ftsZ bw-insert
 
-
|lox71-ftsZ PCR product P7
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|lox71-ftsZ(unmutated) ready for use as a backward insert
 
-
|
 
-
|24
 
-
|[[Paris/July 30| July 30]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D28
 
-
|RBS-DapAColi BB
 
-
|P8 (RBS-DapAColi)
 
-
|EcoRI
 
-
|PstI
 
-
|~950
 
-
|Biobrick
 
-
|
 
-
|54
 
-
|[[Paris/July 25| July 25]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D29
 
-
|RBS-DapAColi
 
-
|P8 (RBS-DapAColi)
 
-
|XbaI
 
-
|PstI
 
-
|~950
 
-
|Backward insert
 
-
|
 
-
|44
 
-
|[[Paris/July 25| July 25]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D30
 
-
|RBS-DapASubtilis BB
 
-
|P9 (RBS-DapASubtilis)
 
-
|EcoRI
 
-
|PstI
 
-
|~950
 
-
|Biobrick
 
-
|
 
-
|72
 
-
|[[Paris/July 25| July 25]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D31
 
-
|RBS-DapASubtilis
 
-
|P9 (RBS-DapASubtilis)
 
-
|XbaI
 
-
|PstI
 
-
|~950
 
-
|Backward insert
 
-
|
 
-
|51
 
-
|[[Paris/July 25| July 25]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D32
 
-
|Lox71-FtsA-FtsZ
 
-
|P10 Lox71-FtsA-FtsZ
 
-
|XbaI
 
-
|PstI
 
-
|~2600
 
-
|Backward insert - EcoRI site still presents : we can't use EcoRI
 
-
|
 
-
|47
 
-
|[[Paris/July 25| July 25]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D33
 
-
|RBS(B0030)CreORF
 
-
|L6' (clone 1 & 2)
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|RBS-Cre digestion for use as a BI
 
-
|
 
-
|29&27
 
-
|[[Paris/July 30| July 30]],[[Paris/August 13| August 13]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D34
 
-
|lox66
 
-
|L9' lox66
 
-
|SpeI
 
-
|PstI
 
-
|
 
-
|Biobrick plasmid carrying lox66 digested as a BV
 
-
|
 
-
|18
 
-
|[[Paris/July 30| July 30]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D35
 
-
|lox71
 
-
|L10' lox71
 
-
|SpeI
 
-
|PstI
 
-
|
 
-
|Biobrick plasmid carrying lox71 digested as a BV
 
-
|
 
-
|
 
-
|[[Paris/July 30| July 30]],[[Paris/August 13| August 13]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D36
 
-
|lox71-KmR-lox66
 
-
|P11 lox71-KmR-lox66
 
-
|EcoRI
 
-
|PstI
 
-
|
 
-
|lox71-KmR-lox66 digested as a biobrick insert
 
-
|
 
-
|
 
-
|[[Paris/July 30| July 30]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D37
 
-
|lox71-gfp(tripart) BI
 
-
|L12.1 & L12.2 miniprep products (from [[Paris/July 30| 30/07]] ligation reaction)
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|lox71_RBS-gfp-double terminator extracted as a backward insert
 
-
|
 
-
|
 
-
|[[Paris/August 6 | Aug. 6 ]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D38
 
-
|BB-insert lox66-DapAE.coli
 
-
|lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2)
 
-
|EcoRI
 
-
|PstI
 
-
|
 
-
|lox66-dapAE.coli extracted from pTOPO plasmid as a BB insert
 
-
|
 
-
|
 
-
|[[Paris/August 6| Aug. 6]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D39'
 
-
|BI-insert lox66-DapAE.coli
 
-
|lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2)
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|lox66-dapAcoli extracted for usage as a backward insert
 
-
|
 
-
|
 
-
|[[Paris/August 7 | Aug. 7 ]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D40
 
-
|BB-insert lox71-KmR-lox66
 
-
|L25.1 miniprep product (from ligation rxn of [[Paris/July 30| 30/07]]
 
-
|EcoRI
 
-
|PstI
 
-
|
 
-
|lox71-KmR-lox66 construct extracted for clonong as a biobrick
 
-
|
 
-
|
 
-
|[[Paris/August 6 | Aug. 6 ]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;" |D41
 
-
|araC/pBad-J61002 plasmid SP dig.
 
-
|L1.2, L1".1 & L1".2 miniprep product
 
-
|SpeI
 
-
|PstI
 
-
|
 
-
|J61002 plasmid containing araC/pBad promotor digested for insertion of a BI CDS
 
-
|
 
-
|
 
-
|[[Paris/August 13 | Aug. 13 ]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;" |D42
 
-
|Lox66 FV
 
-
|L9
 
-
|EcoRI
 
-
|XbaI
 
-
|
 
-
|pSB1A2-lox66 as a Forward vector
 
-
|
 
-
|
 
-
|[[Paris/August 13| August 13]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D43
 
-
|gfp-tripart FI
 
-
|MP8.1
 
-
|EcoRI
 
-
|SpeI
 
-
|
 
-
|gfp-tri part (strongRBS-ORF-T) ready for use as a forward insert
 
-
|
 
-
|
 
-
|[[Paris/August 13| August 13]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D44
 
-
|<bbpart>B0015</bbpart> terminator BI
 
-
|<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.3 Aug.14)
 
-
|XbaI
 
-
|Pst1
 
-
|
 
-
|terminator B0015 extracted for use as a BI
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D45
 
-
|<bbpart>B0015</bbpart> terminator FI
 
-
|<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.4 Aug.14)
 
-
|EcoRI
 
-
|SpeI
 
-
|
 
-
|terminator B0015 extracted for use as a FI
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;" |D46
 
-
|<bbpart>B0015</bbpart> BV
 
-
|<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> (MP3.3 Aug.14)
 
-
|SpeI
 
-
|Pst1
 
-
|
 
-
|<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> plasmid extracted for use as a BV
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;" |D47
 
-
|<bbpart>pSB1A2</bbpart> XP dig.
 
-
|<bbpart>pSB1A2</bbpart> carrying Cre ORF (MP9.1)
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|<bbpart>pSB1A2</bbpart> open vector ready for cloning of BI
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;" |D48
 
-
|<bbpart>pSB1A2</bbpart> ES dig.
 
-
|<bbpart>pSB1A2</bbpart> carrying Cre ORF (MP9.2)
 
-
|EcoRI
 
-
|SpeI
 
-
|
 
-
|<bbpart>pSB1A2</bbpart> open vector ready for cloning of FI
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D49
 
-
|lox71-gfptripart BI
 
-
|lox71-gfptripart in <bbpart>pSB1A2</bbpart> (L12.2)
 
-
|XbaI
 
-
|Pst1
 
-
|
 
-
|lox71-gfptripart extracted for use as a BI
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;" |D50
 
-
|<bbpart>BBa_J61002</bbpart> pTet SP dig.
 
-
|<bbpart>BBa_J61002</bbpart> (MP13.1 [[Paris/August 14| Aug. 14]])
 
-
|SpeI
 
-
|PstI
 
-
|
 
-
|<bbpart>BBa_J61002</bbpart> pTet promoter vector ready for insertion of CDS as a BI
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D51
 
-
|RBS-dapAcoli BBinsert
 
-
|pTOPO of RBS-dapAcoli (MPT4.3)
 
-
|EcoRI
 
-
|Pst1
 
-
|
 
-
|RBS-dapAcoli extracted for cloning as a biobrick
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D52
 
-
|RBS-dapAcoli BI
 
-
|pTOPO of RBS-dapAcoli (MPT4.3)
 
-
|XbaI
 
-
|Pst1
 
-
|
 
-
|RBS-dapAcoli extracted for use as a BI
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D53
 
-
|RBS-dapAsubtilis BBinsert
 
-
|pTOPO of RBS-dapAsubtilis (MPT5.1)
 
-
|EcoRI
 
-
|Pst1
 
-
|
 
-
|RBS-dapAsubtilis extracted for cloning as a biobrick
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D54
 
-
|RBS-dapAsubtilis BI
 
-
|pTOPO of RBS-dapAsubtilis (MPT5.1)
 
-
|XbaI
 
-
|Pst1
 
-
|
 
-
|RBS-dapAsubtilis extracted for use as a BI
 
-
|Ø (24/09/07)
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D55
 
-
|RBS-dapAsubtilis FI
 
-
|pTOPO of RBS-dapAsubtilis (MPT5.2)
 
-
|EcoRI
 
-
|SpeI
 
-
|
 
-
|RBS-dapAsubtilis extracted for use as a FI
 
-
|
 
-
|
 
-
|[[Paris/August 16| August 16]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D57
 
-
|lox71-B0015(T) BI
 
-
|(L31.1, L31.3) lox71-B0015(T) <bbpart>pSB1A2</bbpart>
 
-
|XbaI
 
-
|Pst1
 
-
|
 
-
|lox71-B0015(T) extracted for use as a BI <bbpart>pSB1A2</bbpart>
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;" |D58
 
-
|B0015(T)-lox66 BV
 
-
|(L32.1, L32.2) B0015(T)-lox66 BV
 
-
|SpeI
 
-
|PstI
 
-
|
 
-
|B0015(T)-lox66 for use as a BV <bbpart>pSB1A2</bbpart>
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D59
 
-
|pTet>>lox71-gfptripart BI
 
-
|(L34.1, L34.2) pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|pTet>>lox71-gfptripart for use as a BI
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;"  |D61
 
-
|lox66-mRFP FV
 
-
|(L36.1, L36.2) lox66-mRFP
 
-
|EcoRI
 
-
|XbaI
 
-
|
 
-
|lox66-mRFP part (strongRBS-ORF-T) ready for use as a forward vector
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D62
 
-
|lox66-RBSDapAcoli BI
 
-
|(L37.1, L37.2) lox66-RBSDapAcoli
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|lox66-RBSDapAcoli ready for use as a Backward insert
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D63
 
-
|RBSDapAcoli BI
 
-
|(L39.1, L39.2) RBSDapAcoli
 
-
|XbaI
 
-
|PstI
 
-
|
 
-
|RBSDapAcoli BI (for insertion behind T-lox66)
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;" |D64
 
-
|FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> BV
 
-
|(MP14.1) <bbpart>BBa_J61025</bbpart>
 
-
|SpeI
 
-
|PstI
 
-
|
 
-
|FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> (for backward introduction of a casette for Wanner K insertion)
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;" |D65
 
-
|FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> FV
 
-
|(MP14.1) <bbpart>BBa_J61025</bbpart>
 
-
|EcoR
 
-
|XbaI
 
-
|
 
-
|FRT-CmR-FRT <bbpart>BBa_J61025</bbpart> (for forward introduction of a casette for Wanner K insertion)
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;" |D68
 
-
|attB BV
 
-
|(MP15.1)
 
-
|SpeI
 
-
|PstI
 
-
|
 
-
|vector containing attB for use as a BV
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;" |D69
 
-
|gfptripart-lox66 BV
 
-
|(L29.1) gfptripart-lox66 <bbpart>pSB1A2</bbpart>
 
-
|SpeI
 
-
|PstI
 
-
|
 
-
|
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D70
 
-
|pTet>>lox71-gfptripart FI
 
-
|(L34.1, L34.2) pTet>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
 
-
|EcoRI
 
-
|SpeI
 
-
|
 
-
|pTet>>lox71-gfptripart for use as a FI
 
-
|
 
-
|
 
-
|[[Paris/September 14| Sep. 14]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D71
 
-
|araC/pBad>>lox71-gfptripart FI
 
-
|L35.1 araC/pBad>>lox71-gfptripart <bbpart>BBa_J61002</bbpart>
 
-
|EcoRI
 
-
|SpeI
 
-
|
 
-
|araC/pBad>>lox71-gfptripart for use as a FI
 
-
|Ø (24/09/07)
 
-
|
 
-
|[[Paris/September 14| Sep. 14]][[Paris/September 17| Sep. 17]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;" |D72
 
-
|<bbpart>pSB2K3</bbpart> BBvector
 
-
|MP16 <bbpart>pSB2K3</bbpart>::attP
 
-
|EcoRI
 
-
|PstI
 
-
|
 
-
|Low copy number plasmid, BB vector
 
-
|
 
-
|
 
-
|[[Paris/September 17| Sep. 17]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #FF99FF;" |D73
 
-
|<bbpart>pSB2K3</bbpart> ES open vector
 
-
|MP16 <bbpart>pSB2K3</bbpart>::attP
 
-
|EcoRI
 
-
|SpeI
 
-
|
 
-
|Comparison of digestion efficiency between Pst and Spe in NEB2
 
-
|
 
-
|
 
-
|[[Paris/September 17| Sep. 17]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D76
 
-
|DGAT BB
 
-
|P14 DGAT
 
-
|EcoRI
 
-
|PstI
 
-
|
 
-
|DGAT
 
-
|Ø (24/09/07)
 
-
|
 
-
|[[Paris/September 17| Sep. 17]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;" |D77
 
-
|araC/pBad>>lox71-gfptripart BI
 
-
|D60 (.1 & .2) digestion product
 
-
|FspI
 
-
|AhdI
 
-
|
 
-
|in D60, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion
 
-
|
 
-
|
 
-
|[[Paris/September 17| Sep. 17]]
 
-
|- style="background: #cccccc;" 
 
-
| style="background: #ccffcc;"  |D78
 
-
|araC/pBad>>lox71-gfptripart FI
 
-
|D71 (.1 & .2) digestion product
 
-
|FspI
 
-
|AhdI
 
-
|
 
-
|in D71, the extracted fragment & the open plasmid have the same size, it is thus necessary to remove the plasmid by digestion
 
-
|
 
-
|
 
-
|[[Paris/September 17| Sep. 17]]
 
|}
|}

Revision as of 16:10, 22 July 2008

PCR products

PCR products
Number Name Oligo F Oligo R Matrix Length Comments Product Left ? []ng/µL Date
[[ ]]
[[ ]]


Plasmids: MiniPrep Products

Plasmids: MiniPrep Products
Number Name Biobricks Description Products in use Date
MP1 [http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002] Part icon regulatory.png Strong constitutive promoter in J61002 (clones 1 + 2) 2007
MP2 [http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002] Part icon regulatory.png Medium constitutive promoter in J61002 (clones 1 + 2) MP2.2 2007
MP3 [http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1AK3 pSB1AK3] Part icon terminator.pngPart icon terminator.png Double terminator (clones 1 + 2) MP3.3, MP3.4 2007
MP4 [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] Part icon regulatory.png AraC pBAD (clones 1 + 2) 2007
MP5 [http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Part icon rbs.png Strong RBS (clones 1 + 2) 2007
MP6 [http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.pngPart icon terminator.png ECFP (RBS+LVA+Term) (clones 1 + 2) MP6'.1, MP6'.2 2007
MP8 [http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.pngPart icon terminator.png gfp tri-part; strong rbs (clones 1 + 2) 2007


Plasmids: MiniPrep "in preparation"
Number Name Biobricks Description Products in use Date
[http://partsregistry.org/Part:BBa_B0034 B0034] Part icon rbs.png RBS 2008
[http://partsregistry.org/Part:BBa_C0040 C0040] Icon coding.png tetR repressor 2008
[http://partsregistry.org/Part:BBa_C0079 C0079] Icon coding.png lasR activator 2008
[http://partsregistry.org/Part:BBa_C0179 C0179] Icon coding.png lasR activator 2007
[http://partsregistry.org/Part:BBa_E0030 E0030] Part icon reporter.png YFP 2008
[http://partsregistry.org/Part:BBa_E0040 E0040] Part icon reporter.png GFP 2008
[http://partsregistry.org/Part:BBa_E1010 E1010] Part icon reporter.png mRFP 2008
[http://partsregistry.org/Part:BBa_J23101 J23101] Part icon regulatory.png constitutive promoter member family 2008
[http://partsregistry.org/Part:BBa_J23109 J23109] Part icon regulatory.png constitutive promoter member family 2008
[http://partsregistry.org/Part:BBa_R0040 R0040] Part icon regulatory.png tetR repressible promoter 2008
[http://partsregistry.org/Part:BBa_R0079 R0079] Part icon regulatory.png promoter (lasr & PAI regulated) 2008
[http://partsregistry.org/Part:BBa_S03154 S03154] Part icon rbs.pngIcon coding.png intermediate construction 2008
[http://partsregistry.org/Part:BBa_S03879 S03879] Part icon rbs.pngIcon coding.png intermediate construction 2008


Digestion Products


PURPLE = Vector
GREEN = Insert

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description Product Left []ng/µL Date
D1 <bbpart>pJ23100</bbpart> <bbpart>pJ23100</bbpart> Spe1 Pst1 2096 Vector with the J23100 promoter ready for insertion of a RBS/CDS insert Ø (24/09/07) 34 July 17
D2 <bbpart>mRFP dblTer</bbpart> <bbpart>pJ23100</bbpart> Spe1 Pst1 887 27 July 17
Retrieved from "http://2008.igem.org/Team:Paris/Notebook/Freezer"