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Team:Paris/Notebook/Freezer
From 2008.igem.org
PCR Products
| PCR products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Name | Oligo F | Oligo R | Matrix | Length | Comments | Product Left ? | []ng/µL | Date |
| P1 | Lox71-FtsA-FtsZ-1 | O3 Lox71-FtsA-F | O4 DEcoR1-FtsZ-R | MG1655 | 1537 | Ø | |||
| P2 | FtsZ-2 | O5 DEcoR1-FtsZ-F | O2 FtsZ-R | MG1655 | 1088 | Ø | |||
| P3 | DGAT-1 | 27 ForDGAT1 | 13 DPst1-DGAT-R | Miniprep pKS::DGAT | 1087 | July 31 | |||
| P4 | DGAT-2 | 12 DPst1-DGAT | 27 Rev-DGAT1 | Miniprep pKS::DGAT | 396 | July 31 | |||
| P5 | Lox66-DapAColi | O6 Lox66-DapAColi-F | O7 DapAColi-R | MG1655 | 985 | Ø | July 17, July 30 | ||
| P6 | Lox66-DapASubtilis | O8 Lox66-DapASubt-F | O9 DapASubt-R | B.Subtilis 168 | 979 | July 17, July 27, July 30 | |||
| P7 | Lox71-FtsZ | O1 Lox71-FtsZ-F | O2 FtsZ-R | MG1655 | 1260 | EcoRI site in FtsZ not mutated | July 17, July 30 | ||
| P8 | RBS-DapAColi | O20 RBS-DapAColi-F | O7 DapAColi-R | MG1655 | 952 | B0030-DapAColi | July 23, July 30 | ||
| P9 | RBS-DapASubtilis | O21 RBS-DapASubt-F | O9 DapASubt-R | B.Subtilis 168 | 946 | B0030-DapASubtilis | July 23, July 27 | ||
| P10 | Lox71-FtsA-FtsZ | O3 Lox71-FtsA-F | O2 FtsZ-R | MG1655 | 2580 | EcoRI site in FtsZ not mutated | July 23 | ||
| P11 | Lox71-KmR-Lox66 | O10 Lox71-KmR-F | O11 Lox66-KmR-R | MP12 | 1084 | July 27, July 30 | |||
| P13 | DGAT deltaPst-1 | ForDGAT1 | Megaprimer DGAT2 | DNA plasmid (pKS::DGAT): | 1443 | August 1 | |||
| P14 | DGAT deltaPst-1 biobrick | ForDGAT1bb | RevDGAT1bb | DGAT deltaPst1 | 1504 | August 2 Oct. 18 | |||
| P15 | PacI [pTet>>lox-GFP-loxmRFP] XmaI | O47 | O48 | [pTet>>lox-GFP-loxmRFP] L59.6 | 2kb | August 2 | |||
| P16 | PrefixBB-frt1-CmR-frt2-SufixBB | O51 | O52 | pY-2C-IntC | 1kb | Oct. 15 | |||
| P17 | PrefixBB-frt1-CmR-frt2-T1T2-SufixBB | O51 | O53 | pY-2C-IntC | >1kb | Oct. 18 | |||
Plasmids : MiniPrep Products
| Plasmids: MiniPrep Products | ||||
|---|---|---|---|---|
| Number | Name | Description | Products in use | Date |
| MP1 | pJ23100 in J61002 | Strong constitutive promoter in J61002(clones 1 + 2) 
| July 15, 2007; Aug. 17, 2007 | |
| MP2 | pJ23107 in J61002 | Medium constitutive promoter in J61002 (clones 1 + 2)
| MP2.2 | July 15, 2007; Aug. 17, 2007 |
| MP3 | B0015 in pSB1AK3 | Double terminator (clones 1 + 2) 
| MP3.3, MP3.4 | August 14, 2007 |
| MP4 | I0500 in pSB2K3 | AraC pBAD (clones 1 + 2)
| July 16, 2007 | |
| MP5 | B0030 in pSB1A2 | Strong RBS (clones 1 + 2) 
| July 18, 2007 | |
| MP6 | E0422 in pSB1A2 | ECFP (RBS+LVA+Term) (clones 1 + 2) 
| July 18, 2007; July 24, 2007 | |
| MP7 | E0241 in pSB1A2 | PoPs to GFP converter (clones 1 + 2)
| MP7'.1 | July 18, 2007; July 24, 2007 |
| MP8 | <bbpart>BBa_E0840</bbpart> in <bbpart>pSB1A2</bbpart> | gfp tri-part; strong rbs (clones 1 + 2) <partinfo>E0840 SpecifiedComponents</partinfo> | July 18 | |
