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Team:Paris/Notebook/Freezer
From 2008.igem.org
PCR products
| PCR products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Name | Oligo F | Oligo R | Matrix | Length | Comments | Product Left ? | []ng/µL | Date |
| [[ ]] | |||||||||
| [[ ]] | |||||||||
Plasmids: MiniPrep Products
| Plasmids: MiniPrep Products | |||||
|---|---|---|---|---|---|
| Number | Name | Biobricks | Description | Products in use | Date |
| MP1 | pJ23100 in J61002 | 
| Strong constitutive promoter in J61002 (clones 1 + 2) | 2007 | |
| MP2 | pJ23107 in J61002 |
| Medium constitutive promoter in J61002 (clones 1 + 2) | MP2.2 | 2007 |
| MP3 | B0015 in pSB1AK3 | 
| Double terminator (clones 1 + 2) | MP3.3, MP3.4 | 2007 |
| MP4 | I0500 in pSB2K3 |
| AraC pBAD (clones 1 + 2) | 2007 | |
| MP5 | B0030 in pSB1A2 | 
| Strong RBS (clones 1 + 2) | 2007 | |
| MP6 | E0422 in pSB1A2 | 
| ECFP (RBS+LVA+Term) (clones 1 + 2) | MP6'.1, MP6'.2 | 2007 |
| MP8 | E0840 in pSB1A2 |
| gfp tri-part; strong rbs (clones 1 + 2) | 2007 | |
| Plasmids: MiniPrep "in preparation" | |||||
|---|---|---|---|---|---|
| Number | Name | Biobricks | Description | Products in use | Date |
| B0034 |
| RBS | 2008 | ||
| C0040 | 
| tetR repressor | 2008 | ||
| C0079 |
| lasR activator | 2008 | ||
| C0179 |
| lasR activator | 2007 | ||
| E0030 |
| YFP | 2008 | ||
| E0040 |
| GFP | 2008 | ||
| E1010 |
| mRFP | 2008 | ||
| J23101 |
| constitutive promoter member family | 2008 | ||
| J23109 |
| constitutive promoter member family | 2008 | ||
| R0040 |
| tetR repressible promoter | 2008 | ||
| R0079 |
| promoter (lasr & PAI regulated) | 2008 | ||
| S03154 |
| intermediate construction | 2008 | ||
| S03879 |
| intermediate construction | 2008 | ||
Digestion Products
PURPLE = Vector
GREEN = Insert
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description | Product Left | []ng/µL | Date |
| D1 | <bbpart>pJ23100</bbpart> | <bbpart>pJ23100</bbpart> | Spe1 | Pst1 | 2096 | Vector with the J23100 promoter ready for insertion of a RBS/CDS insert | Ø (24/09/07) | 34 | July 17 |
| D2 | <bbpart>mRFP dblTer</bbpart> | <bbpart>pJ23100</bbpart> | Spe1 | Pst1 | 887 | 27 | July 17 | ||
