Ligations: Constructions realised
|
Name/N°
| Plasmid
| Strain
| Miniprep
| Description
| Digestion
| comment
| Date
|
I0500
| pSB2K3
| S103
| MP119
|  araC/pBad
|
|
| July 24, July 26
|
E0840
| pSB1A2
| S106
| MP122
|     GFP generator; strong rbs
|
|
| July 26
|
B0034
| pSB1A2
| S107
| MP100
|  Strongest RBS (Efficiency = 1)
|
|
| July 26
|
R0079
| pSB1A2
| S108
| MP103
|  pLas (lasr & PAI regulated)
|
|
| July 23, July 26
|
J23101
| J61002
| S109
| MP101
|  Strongest Constitutive promoter
|
|
| July 22, July 23, July 26
|
J23109
| J61002
| S110
| MP102
|  Weak Constitutive promoter
|
|
| July 22, July 26
|
R0040
| pSB1A2
| S111
| MP104
|  pTetR repressible
|
|
| July 23, July 26
|
S03154
| pSB1A2
| S112
| MP105
|   B0034(rbs) ->LasI
|
|
| July 26
|
S03879
| pSB1A2
| S113
| MP106
|   B0034(rbs) -> TetR
|
|
| August 24
|
C0079
| pSB2K3
| S114
| MP107
|  lasR activator with LVA Tag
|
|
| July 23
|
C0179
| pSB1A2
| S115
| MP108
|  lasR activator
|
|
| July 22, July 23, July 26
|
E0030
| pSB1AK3
| S116
| MP109
|  YFP
|
|
| July 26
|
E0040
| pSB1A2
| S117
| MP110
|  GFP
|
|
| July 23, July 26
|
E1010
| pSB2K3
| S118
| MP111
|  mRFP
|
|
| July 23, July 26
|
OmpR* M57I
| Strain
| S119
|
| Strain MG1655
 OmpR*
|
| Really mutated?
| July 23
|
EnvZ* I281S
| Strain
| S120
|
| Strain MG1655
 EnvZ*
|
|
| July 23
|
C0040
| pSB1A2
| S121
| MP114
|  tetR repressor
|
|
| July 23
|
pSB1A3
| pSB1A3
| S122
| MP115
|  plasmid
|
|
| July 23
|
L102
| pSB1A2
| S123
| MP124
|   Strong rbs - YFP
| D129 (BV) - D118 (BI)
|
| August 1
|
L103
| pSB1A2
| S124
| MP125
|   Strong rbs - mRFP
| D129 (BV) - D122 (BI)
|
| August 1
|
L104
| pSB1A2
| S125
| MP126
|   Strong rbs - lasR activator
| D129 (BV) - D114 (BI)
|
| August 1
|
L105
| J61002
| S126
| MP127
|      Strong promoter - ECFP
| D123 (BV) - D130 (BI)
|
| August 1
|
L106
| J61002
| S127
| MP128
|      Strong promoter - GFP Tripart
| D123 (BV) - D131 (BI)
|
| August 1
|
L107
| J61002
| S128
| MP129
|      Strongest promoter - ECFP
| D103 (BV) - D130 (BI)
|
| August 1
|
L108
| J61002
| S129
| MP130
|      Strongest promoter - GFP Tripart
| D103 (BV) - D131 (BI)
|
| August 1
|
L110
| J61002
| S130
| MP131
|      Medium promoter - GFP Tripart
| D124 (BV) - D131 (BI)
|
| August 1
|
L111
| J61002
| S131
| MP132
|      Weak promoter - ECFP
| D104 (BV) - D130 (BI)
|
| August 1
|
L112
| J61002
| S132
| MP133
|      Weak promoter - GFP tripart
| D104 (BV) - D131 (BI)
|
| August 1
|
L115
| pSB1A2
| S133
| MP134
|      pLas - ECFP
| D105 (BV) - D130 (BI)
|
| August 1
|
L116
| pSB1A2
| S134
| MP135
|      pLas - gfp Tripart
| D105 (BV) - D131 (BI)
|
| August 1
|
L117
| pSB1AK3
| S135
| MP136
|    YFP - Double Terminator
| D125 (FV) - D117 (FI)
|
| August 1
|
L118
| pSB1AK3
| S136
| MP137
|    mRFP - Double Terminator
| D125 (FV) - D121 (FI)
|
| August 1
|
L119
| pSB1AK3
| S137
| MP138
|    lasR activator + LVA - Double Terminator
| D125 (FV) - D113 (FI)
|
| August 1
|
L121
| pSB1A2
| S138
| MP139
|      pTet - GFP tripart
| D106 (BV) - D131 (BI) In Top10 strain
|
| August 1
|
L124
| pSB1A2
| S139
| MP140
|   Strongest RBS - mRFP
| D102 (BV) - D122 (BI)
|
| August 1
|
L125
| pSB1A2
| S140
| MP141
|   Strongest RBS - YFP
| D102 (BV) - D118 (BI)
|
| August 1
|
MG1655
| Strain
| S141
|
| Strain
|
|
| Aug 7
|
J61002
| J61002
| S142
| MP123
|  plasmid
|
|
| August 5
|
L128
| J61002
| S143
| MP144
|  pFlgA
| D132 (FI) - D136 (FV)
| OK sequencing
| August 12
|
L100
| pSB1A2
| S146
| MP147
|       rbs TetR - ECFP
| D110 (BV) - D130 (BI)
|
| August 13
|
L114
| pSB2K3
| S148
| MP149
|      araC/pBad - GFP tripart
| D126 (BV) - D131 (BI)
|
| August 13
|
L120
| pSB1A2
| S149
| MP150
|      pTet - ECFP
| D106 (BV) - D130 (BI)
|
| August 13
|
L122
| pSB1A2
| S150
| MP151
|       RBS-lasI - ECFP
| D107 (BV) - D130 (BI)
|
| August 13
|
L123
| pSB1A2
| S151
| MP152
|       RBS lasI - GFP tripart
| D107 (BV) - D131 (BI)
|
| August 13
|
L126
| pSB1A2
| S152
| MP153
|   Strongest RBS (1)- LasR activator (+LVA)
| D102 (BV) - D114 (BI)
|
| August 13
|
L132
| pSB1A2
| S153
| MP154
|  flhDC
| D142(FV) - D139(FI)
|
| August 13
|
L133
| pSB1A2
| S154
| MP155
|  OmpR*
| D142(FV) - D140(FI)
| WT sequence
| August 19
|
L134
| pSB1A2
| S155
| MP156
|  EnvZ*
| D142(FV) - D141(FI)
| lacks about 300 bp in EnvZ*
| August 19
|
L138
| pSB3K3
| S156
| MP157
|     GFP generator (E0240)
| D137(FV) - D138(FI)
|
| August 13
|
E0240
| pSB1A2
| S157
| MP143
|     GFP generator
|
|
| August 15
|
pSB3K3
| pSB3K3
| S158
| MP142
|  plasmid
|
| low to medium copy plasmid with p15A ORI
| August 15
|
L141
| pSB1A2
| S160
| MP159
|  flhC
| D142 (ES) - D145 (ES)
| has not been sequenced
| August 15
|
L151
| pSB1AK3
| S162
| MP162
|     rbs-lasR + dbl ter
| D147 (FI) - D125 (FV)
|
| August 19
|
B0032
| pSB1A2
| S163
| MP163
|  medium rbs (efficiency = 0,3)
|
|
| August 20
|
E0422
| pSB1A2
| S164
| MP121 MP164
|     RBS+ ECFP+ LVA+ term
|
|
| July 24, July 26 August 20
|
E0430
| pSB1A2
| S165
| MP165
|     RBS+ YFP+ LVA- term
|
|
| August 20
|
E0432
| pSB1A2
| S166
| MP166
|     RBS+ YFP LVA+ term
|
| BUT be careful , it doesn't work. See registry
| August 20
|
E0420
| pSB1A2
| S167
| MP167
|     RBS+ ECFP LVA- term
|
|
| August 20
|
I732078
| pSB1A3
| S168
| MP168
|     RBS+ mRFP LVA+ term
|
|
| August 20
|
L153
| J61002
| S169
| MP169
|      Strong promoter-rbs-lasR-dble ter
| D123 (BV) - D165 (BI)
|
| August 20
|
L154
| J61002
| S170
| MP170
|      strongest promoter-rbs-lasR-dble ter
| D103 (BV) - D165 (BI)
|
| August 20
|
L121
| pSB1A2
| S171
| not done
|      pTet - gfp Tripart
| D106 (BV) - D131 (BI)
| Same as S138 but in chemically labmade competent MG1655 bacteria. For study of doubling time of population.
| Aug 22
|
L167
| pSB1A2
| S172
| MP171
|      gfp generator(E0240) - pTet
|
|
| August 25
|
L166
| pSB1A2
| S173
| MP172
|   RBS(B0032) - tetR
|
|
| August 25
|
L160
| J61002
| S174
| MP173
|      pFlgA - YFP Tripart (LVA-)
| D166 (FV) - D132 (FI)
| Fluorescence OK
| August 22
|
L161
| J61002
| S175
| MP174
|      pFlgA - YFP Tripart (LVA+)
| D167 (FV) - D132 (FI)
|
| August 22
|
pFlhB
| pCR4Blunt-TOPO
| S178
| MP175
|   pFlhB-RBS (WT)
|
| OK sequencing
| September 04
|
L
| pCR4Blunt-TOPO
| S179
| MP176
|   pFliA-RBS (WT)
|
| pFliA with conserved Pst1 site
| September 04
|
L
| pCR4Blunt-TOPO
| S181
| MP178
|   pFliL-RBS (WT)
|
| OK sequencing
| September 04
|
L
| pCR4Blunt-TOPO
| S180
| MP177
|   pFlhDC-RBS (WT)
|
| OK sequencing
| September 04
|
L
| pCR4Blunt-TOPO
| S182
| MP179
|   RBS(FlhDC) - FlhDC
|
| FlhDC with conserved Pst1 site
| September 04
|
L
| pCR4Blunt-TOPO
| S183
| MP180
|  FliA2
|
| mutated at EcoR1 and Pst1 internal site
| September 04
|
L
| pCR4Blunt-TOPO
| S184
| MP181
|  FliA3
|
| FliA mutated at EcoR1 and Pst1 site
| September 04
|
L
| pCR4Blunt-TOPO
| S185
| MP182
|  EnvZ*
|
| the good one but not mutated for EcoR1 site
| September 04
|
L
| pCR4Blunt-TOPO
| S186
| MP183
|  OmpR WT
|
| OmpR WT not the mutated
| September 04
|
pFlgB
| pCR4Blunt-TOPO
| S187
| MP184
|   pFlgB-RBS (WT)
|
| OK sequencing
| September 04
|
L
| pCR4Blunt-TOPO
| S188
| MP185
|  OmpR WT
|
| OmpR WT not the mutated
| September 04
|
L179
| J61002
| S200
| MP200
|    J23101 + RBS (B0032) + tet R
|
|
| September 24
|
L
| J61002
| S201
| MP201
|    pFlgA(RBS WT)-EnvZ*
|
| probably wrong according to MP208 and MP209 that have been sequenced
| September 24
|
L198
| J61002
| S207
| MP207
|  pFliA
|
| OK sequencing
| October 04
|
L203
| pSB3K3
| S208
| MP208
|    pFlgA(RBS WT)-EnvZ*
|
| in fact lack of pFlgA
| October 04
|
L206
| J61002
| S209
| MP209
|        pFlgA(RBS WT)-EnvZ*-mRFP tripart LVA+
|
| in fact lack of pFlgA
| October 04
|
L209
| pSB3K3
| S210
| MP210
|   pFliL(RBS WT)
|
| OK sequencing
| October 10
|
L
| pSB3K3
| S211
| MP211
|   pFlgA(RBS WT)
|
| OK sequencing
| October 10
|
L208
| pSB3K3
| S212
| MP212
|   pFlhB(RBS WT)
|
| OK sequencing
| October 10
|
L197
| J61002
| S213
| MP213
|   pFlhB(RBS WT)
|
| OK sequencing
| October 10
|
L200
| J61002
| S214
| MP214
|   pFliL(RBS WT)
|
| OK sequencing
| October 10
|
pSB4T5
| pSB4T5
| S215
| MP215
|  plasmid
|
| low copy plasmid with pSC101 ORI
| October 14
|
E0434
| pSB1AK3
| S216
| MP216
|     YFP tripart +AAV
|
| Fluorescence OK
| October 15
|
JM45
| pJM45
| S217
| MP217
|  plasmid
|
| inducible FlhDC in pBad18 (ORI pBR / ampicillin resistance)
| October 24
|
L226
| J61002
| S218
| MP218
|    pFlhB(RBS WT)-EnvZ*
|
|
| October 24
|
L227
| J61002
| S219
| MP219
|    pFliL(RBS WT)-GFP
|
|
| October 24
|
L228
| J61002
| S220
| MP220
|       pFliL(RBS WT)-ECFP tripart (+LVA)
|
|
| October 24
|
L229
| J61002
| S221
| MP221
|    pFlgA(RBS WT)-GFP
|
|
| October 24
|
L232
| J61002
| S222
| MP222
|       pFlgA(RBS WT)-YFP tripart (+AAV)
|
|
| October 24
|
L234
| J61002
| S223
| MP223
|       pFlhB(RBS WT)-mRFP tripart (+LVA)
|
|
| October 24
|
L225
| J61002
| S224
| MP224
|    pFliL(RBS WT)-EnvZ*
|
|
| October 26
|
L233
| J61002
| S225
| MP225
|    pFlhB(RBS WT)-GFP
|
|
| October 26
|
U317
| pU317
| S226
| MP226
|  plasmid
|
| inducible FliA in pBad18 (ORI pBR / ampicillin resistance)
| October 23
|
NEC283
| Strain
| S227
|
| Strain MG1655
|
| Δ FliA Frt
| October 25
|
NEC284
| Strain
| S228
|
| Strain MG1655
|
| Δ FlhD Frt
| October 25
|
NEC285
| Strain
| S229
|
| Strain MG1655
|
| Δ FlgM Frt
| October 25
|
Δ EnvZ :: Cm
| Strain
| S230
|
| Strain MG1655
|
| Δ EnvZ :: Cm
| October 25
|
L216
| pSB3K3
| MP231
| S231
|   araC/pBad-EnvZ*
|
| inducible EnvZ*
| October 27
|
L236
| J61002
| MP232
| S232
|        pFliL(+WT RBS)-EnvZ*-GFP generator
|
|
| October 29
|
L235
| J61002
| MP233
| S233
|        pFlhB(+WT RBS)-EnvZ*-GFP generator
|
|
| October 29
|
J23100
| J61002
| MP1-2007
| MP116
|  Strong constitutive promoter
|
|
| July 26
|
J23107
| J61002
| MP2-2007
| MP117
|  Medium constitutive promoter
|
|
| July 24, July 26
|
B0030
| pSB1A2
| MP5-2007
| MP120
|  Strong RBS (Efficiency = 0,6)
|
|
| July 26
|
B0015
| pSB1AK3
| MP3-2007
| MP118
|   Double terminator
|
|
| July 24, July 26
|
L174
| J61002
| S
| MP202
|
  Strong constitutive promoter-GFP generator
| J23101-E0240
|
| September 26
|
L157
| pSB1AK3
|
|
|   tetR-rbs (B0015)
| D125 (BV) - D162 (BI)
|
| August 23
|
L155
| J61002
|
|
|      strongest promoter-gfp generator
| D164(FV) - D163 (FI)
|
| August 22
|
L156
| pSB1A2
|
|
|      pTet-gfp generator
| D161 (FV) - D163 (FI)
|
| August 22
|