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Team:Paris/Parts/Plastest
From 2008.igem.org
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==Plasmid for promoter Amplification & Measurement== | ==Plasmid for promoter Amplification & Measurement== | ||
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| + | [[Image:RBS+.jpg|thumb|RBS +]] | ||
| + | [[Image:RBS-.jpg|thumb|RBS -]] | ||
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| + | Those plasmids are very useful vectors to amplify promoters and to measure their forces using Standard Promoter Units. | ||
| + | The principle is easy to understand. | ||
| + | The plasmid contains the BioBrick restriction sites (EcoRI, XbaI, SpeI and PstI) followed by the standard GFP Tripart (E0240). | ||
| + | Instead of using a big biobrick containing Promoter, RBS, GFP and Terminators, the Biobrick used for the test is only the Promoter. | ||
| + | This plasmid can be used to amplify a promoter because only the promoter is in the biobrick. | ||
| + | <br><br><br><br><br><br><br><br><br><br> | ||
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| + | ===Oligonucleotides design=== | ||
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| + | Creation of 3 oligonucleotides ([[team:Paris/Notebook/Oligo|O140, O141, O142]]) in order to create two particular plasmids. | ||
===Necessary stuff=== | ===Necessary stuff=== | ||
Revision as of 16:18, 4 August 2008
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