Team:Paris/Project/FIFO

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=='''FIFO'''==
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[[image:FIFObis.jpg|500px|center]]
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<br><br>
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===First Model===
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Strain : double mutant ΔflhDC and ΔflgM  
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[[image:Oscillations1.jpg|500px]]
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if possible tetR :: Tn10
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Strain : double mutant ΔflhDC and ΔflgM (fliA and its promotor is actually on the bacterial chromosome)
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Designed plasmids
Designed plasmids
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<br>
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<center>
{| border="1"  
{| border="1"  
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|align="center"|Generic Name
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|align="center"|'''Generic Name'''
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|align="center"|Construction
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|align="center"|'''Construction'''
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|align="center"|Advancement
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|align="center"|'''Advancement'''
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|-
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|align="center"|FlhDC
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|align="center"|Master regulator
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|align="center"|pTet - FlhDC
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|align="center"|pTet FlhDC
|align="center"|☣
|align="center"|☣
|-
|-
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|align="center"|Reporter
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|align="center"|Reporter1
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|align="center"|pFliL - TetR - GFP
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|align="center"|pFliL - CFP
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|align="center"|☣
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|-
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|align="center"|Reporter2
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|align="center"|pFlgA(B) - YFP
|align="center"|☣
|align="center"|☣
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|}
 
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===Second Model===
 
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[[image:Oscillations2.jpg|500px]]
 
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Strain : mutant ΔflgM (fliA and flhDC and their promotors are actually on the bacterial chromosome)
 
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Designed plasmids
 
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{| border="1"
 
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|align="center"|Generic Name
 
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|align="center"|Construction
 
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|align="center"|Advancement
 
|-
|-
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|align="center"|Reporter
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|align="center"|Reporter3
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|align="center"|pFliL - TetR - GFP
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|align="center"|pFlhB - RFP
|align="center"|☣
|align="center"|☣
|}
|}
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</center>

Latest revision as of 06:47, 30 October 2008

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FIFObis.jpg



Strain : double mutant ΔflhDC and ΔflgM if possible tetR :: Tn10

Designed plasmids

Generic Name Construction Advancement
Master regulator pTet – FlhDC
Reporter1 pFliL - CFP
Reporter2 pFlgA(B) - YFP
Reporter3 pFlhB - RFP