Team:Paris/Project/FIFO

From 2008.igem.org

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=='''FIFO'''==
=='''FIFO'''==
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===First Model===
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[[image:FIFO.jpg|500px]]
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[[image:Oscillations1.jpg|500px]]
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Strain : double mutant ΔflhDC and ΔflgM (fliA and its promotor is actually on the bacterial chromosome)
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 +
Strain : double mutant ΔflhDC and ΔflgM
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if possible tetR :: Tn10
Designed plasmids
Designed plasmids
{| border="1"  
{| border="1"  
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|align="center"|Advancement
|align="center"|Advancement
|-
|-
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|align="center"|FlhDC
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|align="center"|Master regulator
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|align="center"|pTet - FlhDC
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|align="center"|pTet – FlhDC
|align="center"|☣
|align="center"|☣
|-
|-
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|align="center"|Reporter
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|align="center"|Reporter1
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|align="center"|pFliL - TetR - GFP
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|align="center"|pFliL - GFP
 +
|align="center"|☣
 +
|-
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|align="center"|Reporter2
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|align="center"|pFlgA(B) - RFP
|align="center"|☣
|align="center"|☣
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|}
 
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-
 
-
 
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===Second Model===
 
-
 
-
 
-
 
-
[[image:Oscillations2.jpg|500px]]
 
-
 
-
 
-
Strain : mutant ΔflgM (fliA and flhDC and their promotors are actually on the bacterial chromosome)
 
-
 
-
Designed plasmids
 
-
{| border="1"
 
-
|align="center"|Generic Name
 
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|align="center"|Construction
 
-
|align="center"|Advancement
 
|-
|-
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|align="center"|Reporter
+
|align="center"|Reporter3
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|align="center"|pFliL - TetR - GFP
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|align="center"|pFlhB - CFP
|align="center"|☣
|align="center"|☣
|}
|}

Revision as of 19:23, 17 July 2008

FIFO

FIFO.jpg

Strain : double mutant ΔflhDC and ΔflgM if possible tetR :: Tn10 Designed plasmids

Generic Name Construction Advancement
Master regulator pTet – FlhDC ☣
Reporter1 pFliL - GFP ☣
Reporter2 pFlgA(B) - RFP ☣
Reporter3 pFlhB - CFP ☣
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