Team:Paris/September 12

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Checking our ligases (following)

Analysis of the ligation products by electrophosesis

Ligation of the plasmid digested by EcoRI
Ligation of the plasmid digested by BspHI

1% agarose gel

Analysis of the ligation of the plasmid digested by EcoRI No heat inactivation of the ligase

well n° 1 2 3 4 5 6 7 8 9 10 11 12 13
sample 1 kb ladder MP101 MP101 D202 D202 1 2 3 4 5 6 7 100 bp ladder
purification
digestion products
gel extraction column no
purification
ligase used L1 L2 L3 L1 L2 L3 L1
amount loaded 10 µL 0,5 µL 1 µL 2 µL 3 µL 10 µL 3 µL 10 µL