Revision as of 21:09, 19 July 2008

Friday, July 18th

David Ouyang
- Miniprep R0040 (ptet)
- Digest Amber Suppressor and ptet
- Ligate together
- Do ARFP mutant quikchange and DPN1
- Transform ARFP and Amber Suppressor supposed
Taylor Stevenson
Repeat PCR from yesterday using these primers (NOTE: to view primers download this link and change extension to .doc (word file)[1]).
1. tried raising annealing temperature to 58*C

(100bp standard, BleoC PCR product, Stf PCR product,Ligation of yesterdays digested and CIPed PCR primers, 1kb standard)

Home	The Team	The Project	Parts Submitted to the Registry	Modeling	Notebook

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+=Friday, July 18th=
 *David Ouyang
 **Miniprep R0040 (ptet)
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 **Do ARFP mutant quikchange and DPN1
 **Transform ARFP and Amber Suppressor supposed
+*Taylor Stevenson
+*Repeat PCR from yesterday using these primers (NOTE: to view primers download this link and change extension to .doc (word file)[https://static.igem.org/mediawiki/2008/0/0d/Phage_round_1_primers.mov]).
+*#tried raising annealing temperature to 58*C
+[[Image:20080718.jpg|400px]]
+(100bp standard, BleoC PCR product, Stf PCR product,Ligation of yesterdays digested and CIPed PCR primers, 1kb standard)
+<BR><BR><BR><BR>
+{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+!align="center"|[[Team:Rice_University|Home]]
+!align="center"|[[Team:Rice_University/Team|The Team]]
+!align="center"|[[Team:Rice_University/Project|The Project]]
+!align="center"|[[Team:Rice_University/Parts|Parts Submitted to the Registry]]
+!align="center"|[[Team:Rice_University/Modeling|Modeling]]
+!align="center"|[[Team:Rice_University/Notebook|Notebook]]
+|}