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Team:The University of Alberta/21 October 2008
From 2008.igem.org
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i set up the following ligations with the gel purified products: | i set up the following ligations with the gel purified products: | ||
pUC57-pTET-BisdA+pTET-BisdB | pUC57-pTET-BisdA+pTET-BisdB | ||
| - | + | pSB1A3+laqIQ-ERE-TetR | |
overnight at 16 degrees C. | overnight at 16 degrees C. | ||
Revision as of 03:18, 29 October 2008
David
i digested:
pUC57-pTET-BisdA with SphI+PstI with CIP treatment pTET-BisdB with XbaI+PstI laqIQ-ERE-TetR with XbaI+PstI
i set up the following ligations with the gel purified products:
pUC57-pTET-BisdA+pTET-BisdB pSB1A3+laqIQ-ERE-TetR
overnight at 16 degrees C.
