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Team:The University of Alberta/24 October 2008
From 2008.igem.org
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i set up ligations for pSB1A3+laqIQ-ERE-TetR-pTET-RFP, pSB1A3+pTET-BisdA-pTET-BisdB, pUC57-laqIQ-ERE-TetR-pTET-RFP+pTET-BisdA-pTET-BisdB overnight at 16 degrees C. | i set up ligations for pSB1A3+laqIQ-ERE-TetR-pTET-RFP, pSB1A3+pTET-BisdA-pTET-BisdB, pUC57-laqIQ-ERE-TetR-pTET-RFP+pTET-BisdA-pTET-BisdB overnight at 16 degrees C. | ||
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| + | ==Winnie== | ||
| + | * got the sequencing back from MBSU, looks ok. | ||
Latest revision as of 03:39, 29 October 2008
David
i digested
laqIQ-ERE-TetR-pTET-RFP with XbaI+PstI,
pTET-BisdA-pTET-BisdB with XbaI+PstI,
pUC57-laqIQ-ERE-TetR-pTET-RFP with SphI+PstI and treated with CIP.
i gel purified the desired products.
i set up ligations for pSB1A3+laqIQ-ERE-TetR-pTET-RFP, pSB1A3+pTET-BisdA-pTET-BisdB, pUC57-laqIQ-ERE-TetR-pTET-RFP+pTET-BisdA-pTET-BisdB overnight at 16 degrees C.
Winnie
- got the sequencing back from MBSU, looks ok.
