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Team:The University of Alberta/26 October 2008
From 2008.igem.org
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I performed a mini-prep of pUC57-ER, subsequently digesting it with XbaI and PstI, gel purifying the fragment and using it one of the following ligations. | I performed a mini-prep of pUC57-ER, subsequently digesting it with XbaI and PstI, gel purifying the fragment and using it one of the following ligations. | ||
| - | I set up ligations for of | + | I set up ligations for of pSB1A3+ER, pSB1A3+laqIQ-ERE-TetR-pTET-RFP, pSB1A3+pTET-BisdA-pTET-BisdB, pUC57-laqIQ-ERE-TetR-pTET-RFP+pTET-BisdA-pTET-BisdB and left them overnight at 16 degrees C. |
i was awesome today. | i was awesome today. | ||
Latest revision as of 03:23, 29 October 2008
Jason
I performed a mini-prep of pUC57-ER, subsequently digesting it with XbaI and PstI, gel purifying the fragment and using it one of the following ligations.
I set up ligations for of pSB1A3+ER, pSB1A3+laqIQ-ERE-TetR-pTET-RFP, pSB1A3+pTET-BisdA-pTET-BisdB, pUC57-laqIQ-ERE-TetR-pTET-RFP+pTET-BisdA-pTET-BisdB and left them overnight at 16 degrees C.
i was awesome today.
David
yesterday's transformations didn't work. not at all. no colonies. what is up with that?
