Team:The University of Alberta/2 October 2008

From 2008.igem.org

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** religate 21+22+23(S/P) AND 24+25(X/P)
** religate 21+22+23(S/P) AND 24+25(X/P)
** sequence ETFA/B with Vf and Vr
** sequence ETFA/B with Vf and Vr
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==David==
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i picked candidate colonies from yesterdays transformations to grow up overnight in LB amp.
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  pTET+BisdA
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  pTET+BisdB

Latest revision as of 03:37, 29 October 2008

  • Tom
    • ETFA/B already have RBS site, they are ready for use
    • setup overnight of 21-25(1-4); ETFA/B (5-6)
    • redo 21-25 ligation tomorrow
    • religate 21+22+23(S/P) AND 24+25(X/P)
    • sequence ETFA/B with Vf and Vr

David

i picked candidate colonies from yesterdays transformations to grow up overnight in LB amp.

 pTET+BisdA
 pTET+BisdB