Team:Tokyo Tech

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Revision as of 02:14, 22 October 2008

Home	The Team	The Project	Parts Submitted to the Registry	Modeling	Notebook	Japanese

Team Tokyo Tech <head> </head> <! *********************************************************************************************> <body lang=JA style='tab-interval:42.0pt;text-justify-trim:punctuation'> <div class="links" > <table class="tab" border="1"><tbody> <tr> <td> <ul> <li><a href="#">Our Project</a><br> <li><a href="#Mechanism">Mechanism</a><br> <li><a href="#strategy">Strategy</a> <ol> <li><a href="#1">Construction</a><br> <li><a href="#2">Basic device</a><br> <li><a href="#3">Development of low pressure inducible promoter</a><br> <li><a href="#4">Genetic toggle switch to implement rewritable function </a><br> </ol> <li><a href="#Our Team">Our Team</a><br> <li><a href="#NoteBook">NoteBook</a><br> </ul> </td> </tr> </tbody></table> </div> <div class=Section1 style='layout-grid:18.0pt'> <! ----------------------------------------------------------------------------------------------> <a name="TOPPAGE"></a> <p class=MsoNormal><b><u><font size=6>Our project</font></u></b></p> <table width="100%" border="0" cellspacing="0" cellpadding="0"> <tr> <td class="a" width="0%">　</td> <td class="a"> <p class=MsoNormal><b><font size=4>We want to make a <i style='mso-bidi-font-style:normal'>Coli Touch!!</font></b></p> <td class="a" width="3%">　</td> </tr> </table> <table width="100%" border="0" cellspacing="0" cellpadding="0"> <tr> <td class="a" width="0%">　</td> <td class="a"> <p class=MsoNormal>We try to construct a bacterial ‘touch panel’ which is colored by the pressure.</p></td> <td class="a" width="3%">　</td> </tr> </table> <table width="100%" border="0" cellspacing="0" cellpadding="0"> <tr> <td class="a" width="0%">　</td> <td class="a"> <p class=MsoNormal>We name it <i>Coli touch.</i></p></td> <td class="a" width="3%">　</td> </tr> </table> <table width="100%" border="0" cellspacing="0" cellpadding="0"> <tr> <td class="a" width="3%">　</td> <td class="a"> <p class=MsoNormal><span lang=EN-US>Until iGEM2007, input style was only heat, small molecule and light. These input style have problems. For example, Heat input occur thermal gradient, small molecule input occur concentration gradient, light input depend on location of a container. So, we thought input style except these. That is “press”. Press input has an advantage that input signal travel uniformly in a container.</span></p></td> <td class="a" width="3%">　</td> </tr> </table> <! ----------------------------------------------------------------------------------------------------------> <a name="Mechanism"></a> <p class=MsoNormal><b><font size=4>Mechanism</font></b></p> <table width="100%" border="0" cellspacing="0" cellpadding="0"> <tr> <td class="a" width="0%">　</td> <td class="a"> <p class=MsoNormal style='text-indent:5.25pt;mso-char-indent-count:.5'><span lang=EN-US>We construct genetic circuits that can respond to pressure signal. Lac promoter was known as a pressure inducible promoter. Lac promoter is repressed by LacI protein. The repression is released by adding IPTG (Isopropyl β-D-1-thiogalactopyranoside). Besides IPTG induction, pressure induction is possible. Under high pressure, the affinity of lac protein for lac operon decreases by transition of Lac protein from tetramer to dimer. Through this mechanism, Lac promoter is induced by pressure. </span></p></td> <td class="a" width="3%">　</td> </tr> </table> <IMG src="https://static.igem.org/mediawiki/2008/b/bd/Test.jpg" alt="[サンプルの絵]" width="300"> <! ----------------------------------------------------------------------------------------------------------> <! strategy> <a name="strategy"></a> <p class=MsoNormal><b><font size=4>Strategy</font></b></p> <table width="100%" border="0" cellspacing="0" cellpadding="0"> <tr> <td class="a" width="0%">　</td> <td class="a"> <p class=MsoNormal>We thought two projects, basic project and application project. Basic project consist of two categories. One is construction of pressure responsive genetic (1). The other is creating basic device for a touch display (2). Application project also consist of two categories. One is development of low pressure inducible promoter (3). The other is construction of pressure responsive toggle switch (4). In the future, we want to create the touch display made of E. coli. </p></td> <td class="a" width="3%">　</td> </tr> </table> <IMG src="https://static.igem.org/mediawiki/2008/c/cf/Test2.jpg" alt="[サンプルの絵]" width="700"> <table width="100%" border="0" cellspacing="0" cellpadding="0"> <tr> <td class="a" width="0%">　</td> <td class="a"> <p class=MsoNormal>We used these equipments (show figure) for pressure experiments. </p></td> <td class="a" width="3%">　</td> </tr> </table> <IMG src="https://static.igem.org/mediawiki/2008/a/a3/Tech_kaatu.jpg" align="middle" width="500"> <! ----------------------------------------------------------------------------------------------------------> <a name="1"></a> <p class=MsoNormal><b><u><font size=4>1.Construction</font></u></b></p> <table width="100%" border="0" cellspacing="0" cellpadding="0"> <tr> <td class="a" width="0%">　</td> <td class="a"> <p class=MsoNormal>We construct these plasmids (show figure). </p></td> <td class="a" width="3%">　</td> </tr> </table> <IMG src="https://static.igem.org/mediawiki/2008/6/63/Tech_constraction.jpg" align="middle"> <table width="100%" border="0" cellspacing="0" cellpadding="0"> <tr> <td class="a" width="0%">　</td> <td class="a"> <p class=MsoNormal>Each part is constructed on low-copy plasmid “pSB6”. </p></td> <td class="a" width="3%">　</td> </tr> </table> <! ----------------------------------------------------------------------------------------------------------> <a name="2"></a> <p class=MsoNormal><b><u><font size=4>2. Basic device</font></u></b></p> <table width="100%" border="0" cellspacing="0" cellpadding="0"> <tr> <td class="a" width="0%">　</td> <td class="a"> <p class=MsoNormal>We created pressurizing device for first step of creating touch display. This device has two holes (show figure). Each hole contains tubes and water. Inside tubes E. coli is cultivated. Pressure can travel to inside tubes. One hole (B) is covered with a block made of an acrylic glass. (show figure) Therefore the hole is not pressurized by water. The other (A) is covered with a plastic tape (show figure). Therefore the hole is pressurized. </p></td> <td class="a" width="3%">　</td> </tr> </table> <IMG src="https://static.igem.org/mediawiki/2008/9/9d/Tech_youki.jpg" align="middle" width="500" > <! ---------------------------------------------------------------------------------------------------------> <a name="3"></a> <p class=MsoNormal><b><u><font size=4>3. Development of low pressure inducible promoter </font></u></b></p> <table width="100%" border="0" cellspacing="0" cellpadding="0"> <tr> <td class="a" width="0%">　</td> <td class="a"> <p class=MsoNormal>In the stage of basic project, very high pressure (30MPa) is required for pressure induction of lac promoter. But lower pressure response is needed for creating touch display. Therefore we develop lower pressure inducible promoter. We performed random mutagenesis to lac promoter and tried to screen promoters with FACS.</p></td> <td class="a" width="3%">　</td> </tr> </table> <IMG src="https://static.igem.org/mediawiki/2008/3/3e/Tech_dev1.jpg" align="middle" width="500" > <! ---------------------------------------------------------------------------------------------------------> <a name="4"></a> <p class=MsoNormal><b><u><font size=4>4.Genetic toggle switch to implement rewritable function </font></u></b></p> <table width="100%" border="0" cellspacing="0" cellpadding="0"> <tr> <td class="a" width="0%">　</td> <td class="a"> <p class=MsoNormal>A touch display are required a function of rewritable. Rewritable function consists of writable and erasable one. We adopted a genetic toggle switch that responds to pressure (show figure)</p></td> <td class="a" width="3%">　</td> </tr> </table> <IMG src="https://static.igem.org/mediawiki/2008/5/56/Tech_4toggle1.jpg" align="middle" width="500" > <! ---------------------------------------------------------------------------------------------------------> <a name="Our Team"></a> <p class=MsoNormal><b><font size=4>Our Team</font></b></p> <! ---------------------------------------------------------------------------------------------------------> <a name="NoteBook"></a> <p class=MsoNormal><b><font size=4>NoteBook</font></b></p> </div> </body> </p><div class="printfooter"> Retrieved from "<a href="http://2008.igem.org/Team:Tokyo_Tech">http://2008.igem.org/Team:Tokyo_Tech</a>"</div> <div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div></div>  <div class="visualClear"></div> </div> </div>  <div class="visualClear"></div> <div id='footer-box'> <div id="footer"> <div id="f-poweredbyico"><a href="http://www.mediawiki.org/"><img src="/wiki/skins/common/images/poweredby_mediawiki_88x31.png" height="31" width="88" alt="Powered by MediaWiki" /></a></div> <div id="f-copyrightico"><a href="http://creativecommons.org/licenses/by-sa/3.0/"><img src="http://i.creativecommons.org/l/by-sa/3.0/88x31.png" alt="Attribution-Share Alike 3.0 Unported" width="88" height="31" /></a></div> <ul id="f-list">  <li id="t-recentchanges"><a href="/Special:RecentChanges" title='Recent changes'>Recent changes</a></li> <li id="t-whatlinkshere"><a href="/Special:WhatLinksHere/Team:Tokyo_Tech" title="List of all wiki pages that link here [j]" accesskey="j">What links here</a></li> <li id="t-recentchangeslinked"><a href="/Special:RecentChangesLinked/Team:Tokyo_Tech" title="Recent changes in pages linked from this page [k]" accesskey="k">Related changes</a></li> <li id="t-specialpages"><a href="/Special:SpecialPages" title="List of all special pages [q]" accesskey="q">Special pages</a> </li> <li><a href='/Special:Preferences'>My preferences</a></li> </ul> </div>  <div id='footer'> <ul id="f-list"> <li id="t-print"><a href="/wiki/index.php?title=Team:Tokyo_Tech&diff=60014&oldid=60007&printable=yes" title="Printable version of this page [p]" accesskey="p">Printable version</a> </li> <li id="t-permalink"><a href="/wiki/index.php?title=Team:Tokyo_Tech&oldid=60014" title="Permanent link to this revision of the page">Permanent link</a> </li> <li id="privacy"><a href="/2008.igem.org:Privacy_policy" title="2008.igem.org:Privacy policy">Privacy policy</a></li> <li id="disclaimer"><a href="/2008.igem.org:General_disclaimer" title="2008.igem.org:General disclaimer">Disclaimers</a></li> </ul> </div>  </div>  <script>if (window.runOnloadHook) runOnloadHook();</script> </div> </body> </html>

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