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Team:Tokyo Tech
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Revision as of 14:04, 26 October 2008
| Home | Construction | Acrylic container | Development of promoter | Genetic toggle switch | Parts Submitted to the Registry | Result | Our Team | Acknowledgements |
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Contents |
Our project
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We want to make a Coli Touch!!
To make a Coli Touch, we use input style of pressure. |
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Why pressure?
First, there were various ways as input.
For example, small molecule, heat, and light were used until iGEM 2007.
But no one used pressure as input.
Moreover, past way(small molecule, heat and light) are difficult to induct uniformly.
Pressurize can induct unifomly.It's prospect of technological application in confirmatory experiment. |
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We use pressure sensitive parts.
One paper says "One promoter is sensitive to pressure.". So we focused on it. |
Acrylic container
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We create devices for confirming pressure response of lac promoter. This is the first step of creating touch display. This device made of Acrylic glasses and has two holes (show figure). Each hole contains tubes and water. Inside tubes E. coli is cultivated. Pressure can travel to inside tubes. One hole (A) is covered with a plastic tape (show figure). Therefore the hole is pressurized.The other (B) is covered with a block made of an acrylic glass. (show figure) Therefore the hole is not pressurized by water. |
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E.coli type in tubes |
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* “Ptet” on pSB6 plasmid (E.coli strain; JM109) |
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After pressurized the container, we observed the E.coli by a fluorescence microscope. The result shows below. |
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Aplication project
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About one paper, 94 times activity was observed at 30MPa for the lac
promoter compared with the level expressed at atmospheric pressure.
(cf.Takako Sato, Chiaki Kato,and Koki Horikoshi(1994) Effect of high
pressure on gene expression by lac and tac promoters in Escherichia
coli.Marine Biotechnology 3:89-92)
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Development of low pressure inducible promoter
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In the stage of basic project, very high pressure (30MPa) is required for pressure induction of lac promoter. But lower pressure response is needed for creating touch display. Therefore we develop lower pressure inducible promoter. We performed random mutagenesis to lac promoter and tried to screen promoters with FACS. |
Genetic toggle switch to implement rewritable function
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A touch display are required a function of rewritable. Rewritable function consists of writable and erasable one. We adopted a genetic toggle switch that responds to pressure (show figure) |
