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Team:Tokyo Tech/Genetic toggle switch to implement rewritable function
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1. '''Writable function''' | 1. '''Writable function''' | ||
| - | + | * Plac is induced under 30MPa pressure(PL is weakly induced) | |
| - | + | * Express CI and GFP | |
| - | + | * CI represses PL and causes low LacI concentration | |
| - | + | * Express increasingly CI and GFP ⇒ '''Bright!!''' | |
2. '''Erasable function''' | 2. '''Erasable function''' | ||
| - | + | * PL is induced by heat | |
| - | + | * Express LacI and LacI represses Plac | |
| - | + | * Therefore, GFP expression decreases | |
== Mathematical model == | == Mathematical model == | ||
| + | [[Image:Room_pressure_model.png|frame|right|fig5-2:Room pressure model]] | ||
| + | [[Image:30MPa_pressure_model.png|frame|right|fig5-3:30MPa pressure model]] | ||
Revision as of 21:00, 22 October 2008
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Genetic toggle switch to implement rewritable function
We think two type models - genetic circuit model and mathematical model.
Genetic circuit model
fig5-1:genetic circuit model
1. Writable function
- Plac is induced under 30MPa pressure(PL is weakly induced)
- Express CI and GFP
- CI represses PL and causes low LacI concentration
- Express increasingly CI and GFP ⇒ Bright!!
2. Erasable function
- PL is induced by heat
- Express LacI and LacI represses Plac
- Therefore, GFP expression decreases
Mathematical model
fig5-2:Room pressure model
fig5-3:30MPa pressure model
