Team:Tokyo Tech/Genetic toggle switch to implement rewritable function

From 2008.igem.org

(Difference between revisions)
Line 18: Line 18:
== Genetic circuit model ==
== Genetic circuit model ==
-
[[Image:Tech_4toggle1.JPG]]
+
[[Image:Toggle_genetic_circuit_model.png|frame|center|fig5-1:genetic circuit model]]
-
 
+
-
fig5-1:genetic circuit model
+

Revision as of 21:01, 22 October 2008

Home Construction Acrylic container Development of promoter Genetic toggle switch Parts Submitted to the Registry The Team Notebook


Genetic toggle switch to implement rewritable function

We think two type models - genetic circuit model and mathematical model.

Genetic circuit model

fig5-1:genetic circuit model


1. Writable function

  • Plac is induced under 30MPa pressure(PL is weakly induced)
  • Express CI and GFP
  • CI represses PL and causes low LacI concentration
  • Express increasingly CI and GFP ⇒ Bright!!


2. Erasable function

  • PL is induced by heat
  • Express LacI and LacI represses Plac
  • Therefore, GFP expression decreases

Mathematical model

fig5-2:Room pressure model
fig5-3:30MPa pressure model
Retrieved from "http://2008.igem.org/Team:Tokyo_Tech/Genetic_toggle_switch_to_implement_rewritable_function"