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Team:Tokyo Tech/Genetic toggle switch to implement rewritable function
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= Genetic toggle switch to implement rewritable function= | = Genetic toggle switch to implement rewritable function= | ||
| + | In order to implement rewritable function, we tried to construct Pressure Inducible Genetic Toggle Switch (in short PIGTS). | ||
We think two type models - genetic circuit model and mathematical model. | We think two type models - genetic circuit model and mathematical model. | ||
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[[Image:30MPa_pressure_model.png|frame|right|fig5-3:30MPa pressure model]] | [[Image:30MPa_pressure_model.png|frame|right|fig5-3:30MPa pressure model]] | ||
| - | + | Design of pressure inducible genetic toggle switch(in short PIGTS) | |
We have to choose promoter-RBS strength which satisfy following condition to implement rewritable function. | We have to choose promoter-RBS strength which satisfy following condition to implement rewritable function. | ||
* Converge to high CI concentration state under 30MPa pressure (writable) | * Converge to high CI concentration state under 30MPa pressure (writable) | ||
Revision as of 08:29, 24 October 2008
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Genetic toggle switch to implement rewritable function
In order to implement rewritable function, we tried to construct Pressure Inducible Genetic Toggle Switch (in short PIGTS). We think two type models - genetic circuit model and mathematical model.
Genetic circuit model
fig5-1:genetic circuit model
1. Writable function
- Plac is induced under 30MPa pressure(PL is weakly induced)
- Express CI and GFP
- CI represses PL and causes low LacI concentration
- Express increasingly CI and GFP ⇒ Bright!!
2. Erasable function
- PL is induced by heat
- Express LacI and LacI represses Plac
- Therefore, GFP expression decreases
Mathematical model
fig5-2:Room pressure model
fig5-3:30MPa pressure model
Design of pressure inducible genetic toggle switch(in short PIGTS) We have to choose promoter-RBS strength which satisfy following condition to implement rewritable function.
- Converge to high CI concentration state under 30MPa pressure (writable)
- Bistable under ordinary pressure (erasable)
fig5-4:Desirable condition
