Team:Tokyo Tech/Genetic toggle switch to implement rewritable function
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(Difference between revisions)
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- | + | '''1. Writable function''' | |
* Plac is induced under 30MPa pressure(PL is weakly induced) | * Plac is induced under 30MPa pressure(PL is weakly induced) | ||
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- | + | '''2. Erasable function''' | |
* PL is induced by heat | * PL is induced by heat | ||
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- | We have to choose promoter-RBS strength which satisfy following condition to implement rewritable function | + | We have to choose promoter-RBS strength which satisfy following condition to implement rewritable function. |
# Converge to high CI concentration state under 30MPa (writable) | # Converge to high CI concentration state under 30MPa (writable) | ||
# Bistable under ordinary pressure (erasable) | # Bistable under ordinary pressure (erasable) | ||
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[[Image:Desirable_condition.png|frame|left|fig5-4:Desirable condition]] | [[Image:Desirable_condition.png|frame|left|fig5-4:Desirable condition]] | ||
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Revision as of 05:48, 23 October 2008
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Genetic toggle switch to implement rewritable function
We think two type models - genetic circuit model and mathematical model.
Genetic circuit model
1. Writable function
- Plac is induced under 30MPa pressure(PL is weakly induced)
- Express CI and GFP
- CI represses PL and causes low LacI concentration
- Express increasingly CI and GFP ⇒ Bright!!
2. Erasable function
- PL is induced by heat
- Express LacI and LacI represses Plac
- Therefore, GFP expression decreases
Mathematical model
We have to choose promoter-RBS strength which satisfy following condition to implement rewritable function.
- Converge to high CI concentration state under 30MPa (writable)
- Bistable under ordinary pressure (erasable)
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