Team:Tsinghua

From 2008.igem.org

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Although this page is still in heavy construction, every guest is welcomed to left your trace on the "Doodle Board".
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<div style="background-color:#d2fcd9; color:#d2fcd9">
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            ''' Our Team      Project  Parts      Experiments        Communication Links    Doodle Board'''
 
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Our  Team
 
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[[Image:Girls' Day 4.jpg]]
 
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==Team Members ==
 
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="100%" align="center"
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!align="center"|[[Team:Tsinghua|HOME]]
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!align="center"|[[Team:Tsinghua/Team|Team]]
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!align="center"|[[Team:Tsinghua/Project|Project 1]]
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!align="center"|[[Team:Tsinghua/Project2|Project 2]]
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!align="center"|[[Team:Tsinghua/Parts|Parts]]
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!align="center"|[[Team:Tsinghua/Modelling|Modelling]]
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!align="center"|[[Team:Tsinghua/Notebook|Notebook]]
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!align="center"|[[Team:Tsinghua/Doodle|Doodle Board]]
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|}
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<br>
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[[Image:554.jpg|center|]]
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<br>
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=Our ideas=
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{|align="justify"
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|
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====Poor Driver In a Strange Car====
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      Luying Jia  [[Image:Luying Jia.jpg]]                    Yuanfan Yang
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The car is strange because it can only run forward or tumble. The probability of the two states is controlled through a handle in it. Even worth, there are no windows on this car but a barometer showing local altitude. How should the driver do to reach the top of the hill and stay at there?
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      Gechong Ruan                Shan Lin
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...
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      Xin Rong                      Yicheng Long[[Image:Yicheng Long.jpg]]
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If you think about the design of bacterial chemotaxis pathway, you will find that this pathway just plays the role of the driver in the story above. Interestingly, we find E. coli chemotaxis pathway realizes this delicate dicision-making process through the interactions amount handful molecules. Our project will show how we learn the design principles from native pathway to complete our device.
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      Ziying Liu                      Yuemeng Wang [[Image:Yuemeng Wang.jpg]]
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      Yilong Zou [[Image:Yilong Zou.jpg]]                    Zi Wang[[Image:Zi Wang.jpg]]
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      He Yang[[Image:He Yang.jpg]]
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      Yongqiang Gou                Chao Wang[[Image:Chao Wang.jpg]]
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      Junjie Luo                    Cong Liu[[Image:Cong Liu.jpg]]
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|<html><object width="425" height="350"> <param name="movie"value="http://www.youtube.com/v/EfY4RTA93ME"</center> </param> <embed src="http://www.youtube.com/v/EfY4RTA93ME" type="application/x-shockwave-flash" width="425" height="350"></embed></object></html>
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|}
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Project
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=Introduction of Tsinghua University=
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  This year we are trying to work at the motility of bacteria. E.coli senses some chemicals and moves toward
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<background-color:#ACF8AE; color:#ACF8AE">
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{|align="justify"
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|Tsinghua University was established in 1911, originally under the name “Tsinghua Xuetang”. The school was renamed "Tsinghua School" in 1912. The university section was founded in 1925. The name “National Tsinghua University” was adopted in 1928.[[gallery|click here to see more pictures]]
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or stays still, this behavior is called chemotaxis. Any modification of the pathway from the sensor to the
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The educational philosophy of Tsinghua is to "train students with integrity." Among over 120,000 students who have graduated from Tsinghua since its founding are many outstanding scholars, eminent entrepreneurs and great statesmen remembered and respected by their fellow Chinese citizens.
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motile apparatus will cause into different chemotactic effcts.
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With the motto of “Self-Discipline and Social Commitment” and the spirit of “Actions Speak Louder than Words”, Tsinghua University is dedicated to the well-being of Chinese society and to world development.
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|[[image:thu_1.jpg|right]]
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|}
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Parts
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=News=
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  Jamboree is coming in  8 days!
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  Tsinghua team, Beijing_Normal team, USTC team will lodge together during the Jamboree.
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  last edit by [[User:Ylzouyilong|Ylzouyilong]] Oct 29.2008
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Communication
 
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Experiment and Protocol
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= Communication =
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    1  PCR
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  1.1 Gold Faster Taq system:
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    Reagent           Concentration/Activity 50ul 100ul
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    GF taq buffer        10x                         5      10
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    GF taq                                 0.5   0.5
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    dNTPmix             10mM each                 1   2
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    Primer 1             10uM                 1   2
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    Primer 2             10um                 1   2
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    Template DNA       changeable                 0.5   1
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    MgCl2             0.2M                 0.5   1
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    ddH2O                 ---                 40.5 81
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  1.2 The program under Gold faster taq system
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    Progress     Program I                 Program 2
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    Predenaturing    95℃        2-5 min         95℃  2-5 min
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    Denaturing     95℃        10-20sec         95℃  10-20sec
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    Annealing     (Tm-5) ℃  2-5 sec         68℃  10-15sec/1kb
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    Extension     72℃        10-15sec/1kb
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                  25-30cycle               25-30cycle
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    Last extension   1-2min or skipped       1-2min or skipped
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    2 Restriction cut:
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[[ Communication and Interaction ]]
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            Reagent         Concentration/Activity Volume(50ul system)
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            Restriction cut buffer 10x           5ul
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            Enzyme 1                 --           2.5ul
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            Enzyme 2                 --           2.5ul
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  Incubate at 37℃, 1.5 hrs or longer
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  (Enzymes from Takara Co., Ltd)
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      3  Ligation:
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= Acknowledgement =
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              Reagent     Volume(10ul system)
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[[Image:Tsinghua.gif]][[Image:Biobricks.png]][[Image:SB4.0.gif]][[Image:invitrogen.jpg]][[Image:mathwork.jpg]][[Image:geneart.gif]][[Image:DSM3.GIF]]
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              Solution I 5ul
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="100%" align="center"
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              DNA fragment 3.5ul
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!align="center"|[[Team:Tsinghua|HOME]]
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              Vector         1.5ul
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!align="center"|[[Team:Tsinghua/Team|Team]]
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    Incubate at 16-18℃,1hr or longer
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!align="center"|[[Team:Tsinghua/Project|Project 1]]
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    (Ligation kit from Takara)
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!align="center"|[[Team:Tsinghua/Project2|Project 2]]
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!align="center"|[[Team:Tsinghua/Parts|Parts]]
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!align="center"|[[Team:Tsinghua/Modelling|Modelling]]
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!align="center"|[[Team:Tsinghua/Notebook|Notebook]]
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!align="center"|[[Team:Tsinghua/Doodle|Doodle Board]]
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|}
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<!--- The Mission, Experiments --->
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[[image:06.jpeg|center|]]
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Links
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== Headline text ==
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  Tsinghua University:
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      http://www.tsinghua.edu.cn/qhdwzy/index.jsp
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  Parts:
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      http://partsregistry.org
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  Beijing Normal University:
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      https://2008.igem.org/Team:Beijing_Normal
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  ETH_Zurich:
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      https://2008.igem.org/Team:ETH_Zurich
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  Nature:
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      http://www.nature.com
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  Science:
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      http://www.sciencemag.org
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  System and Synthetic Biology:
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      http://www.springer.com/biomed/journal/11693
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Doodle Board
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  Oh, My E.coli, where are you!        Yilong 08.7.11
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Latest revision as of 04:44, 30 October 2008


HOME Team Project 1 Project 2 Parts Modelling Notebook Doodle Board


554.jpg


Contents

Our ideas

Poor Driver In a Strange Car

The car is strange because it can only run forward or tumble. The probability of the two states is controlled through a handle in it. Even worth, there are no windows on this car but a barometer showing local altitude. How should the driver do to reach the top of the hill and stay at there? ... If you think about the design of bacterial chemotaxis pathway, you will find that this pathway just plays the role of the driver in the story above. Interestingly, we find E. coli chemotaxis pathway realizes this delicate dicision-making process through the interactions amount handful molecules. Our project will show how we learn the design principles from native pathway to complete our device.

Introduction of Tsinghua University

<background-color:#ACF8AE; color:#ACF8AE">

Tsinghua University was established in 1911, originally under the name “Tsinghua Xuetang”. The school was renamed "Tsinghua School" in 1912. The university section was founded in 1925. The name “National Tsinghua University” was adopted in 1928.click here to see more pictures

The educational philosophy of Tsinghua is to "train students with integrity." Among over 120,000 students who have graduated from Tsinghua since its founding are many outstanding scholars, eminent entrepreneurs and great statesmen remembered and respected by their fellow Chinese citizens.

With the motto of “Self-Discipline and Social Commitment” and the spirit of “Actions Speak Louder than Words”, Tsinghua University is dedicated to the well-being of Chinese society and to world development.

Thu 1.jpg

News

 Jamboree is coming in  8 days!
 Tsinghua team, Beijing_Normal team, USTC team will lodge together during the Jamboree.
  last edit by Ylzouyilong Oct 29.2008


Communication

Communication and Interaction

Acknowledgement

Tsinghua.gifBiobricks.pngSB4.0.gifInvitrogen.jpgMathwork.jpgGeneart.gifDSM3.GIF

HOME Team Project 1 Project 2 Parts Modelling Notebook Doodle Board
06.jpeg
== Headline text ==
Retrieved from "http://2008.igem.org/Team:Tsinghua"