Team:Tsinghua

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             Our Team       Project   Parts      Experiments        Communication Links     Doodle Board

Our Team

Team Members

      Luying Jia                      Yuanfan Yang 
      Gechong Ruan                Shan Lin
      Xin Rong                       Yicheng Long
      Ziying Liu                       Yuemeng Wang 
      Yilong Zou                     Zi Wang
      He Yang
      Yongqiang Gou                 Chao Wang
      Junjie Luo                    Cong Liu

Our Schedule

        Time	              Events
 Dec. 15, 2007	              Team of Tsinghua University Founded
 Dec. 31, 2007	              Brain Storm(1)
 Jan-Feb, 2008	              Backgroud Investigation
 Feb.25, 2008	              Brain Storm(2)
 Mar., 2008	              Experimental procedure design;
                             Modeling in silico Started  
 Apr.1,2008	              Wet Lab work started
 Apr.-Aug. ,2008	      Wet Lab Work
 May.1, 2008	              Team Registry
 May.25, 2008	              Students’ Association of Synthetic Biology(SASB) Founded
 June.21, 2008	              “Teach the teacher “workshop, in Kyoto, Japan
 Sept.-Oct., 2008	      Inspection and Revision     
 Oct.20,2008	              Summary, Presentation Preparation
 Nov.8-9,2008	              Jamboree In MIT,

Project

  This year we are trying to work at the motility of bacteria. E.coli senses some chemicals and moves toward

or stays still, this behavior is called chemotaxis. Any modification of the pathway from the sensor to the

motile apparatus will cause into different chemotactic effcts.

Parts

Communication

Experiment and Protocol

   1   PCR
    
 1.1 Gold Faster 	Taq system:
   Reagent	           Concentration/Activity	50ul 	100ul
   GF taq buffer     	    10x	                         5       10
   GF taq		                                 0.5	  0.5
   dNTPmix	            10mM each	                 1	  2
   Primer 1 	            10uM	                 1	  2
   Primer 2	            10um	                 1	  2
   Template DNA	      changeable	                 0.5	  1
   MgCl2	            0.2M	                 0.5	  1
   ddH2O	                ---	                40.5	 81
 1.2 The program under Gold faster taq system
   Progress	     Program I	                 Program 2
   Predenaturing    95℃         2-5 min	         95℃   2-5 min
   Denaturing	     95℃        10-20sec	         95℃   10-20sec
   Annealing	     (Tm-5) ℃   2-5 sec	         68℃   10-15sec/1kb
   Extension	     72℃        10-15sec/1kb

25-30cycle 25-30cycle

   Last extension	  1-2min or skipped	       1-2min or skipped

   2 	Restriction cut:
           Reagent	        Concentration/Activity	 Volume(50ul system)
           Restriction cut buffer	10x	           5ul
           Enzyme 1	                --	           2.5ul
           Enzyme 2	                --	           2.5ul
 Incubate at 37℃, 1.5 hrs or longer
 (Enzymes from Takara Co., Ltd)

     3  Ligation:
              Reagent	    Volume(10ul system)
              Solution I	5ul
              DNA fragment	3.5ul
              Vector	        1.5ul
   Incubate at 16-18℃,1hr or longer
   (Ligation kit from Takara)

Links

 Tsinghua University: 
      http://www.tsinghua.edu.cn/qhdwzy/index.jsp
 Parts: 
      http://partsregistry.org
 Beijing Normal University:
      https://2008.igem.org/Team:Beijing_Normal 
 ETH_Zurich:
      https://2008.igem.org/Team:ETH_Zurich
 Nature:
      http://www.nature.com
 Science:
      http://www.sciencemag.org
 System and Synthetic Biology:
      http://www.springer.com/biomed/journal/11693

Doodle Board

 Oh, My E.coli, where are you!        Yilong 08.7.11