Team:Tsinghua
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Although this page is still in heavy construction, every guest is welcomed to left your trace on the "Doodle Board". | Although this page is still in heavy construction, every guest is welcomed to left your trace on the "Doodle Board". | ||
- | ''' Our Team Project Parts Experiments Communication Links Doodle Board''' | + | ''' Our Team [[Project]] Parts Experiments Communication Links Doodle Board''' |
Our Team | Our Team |
Revision as of 11:29, 28 July 2008
Although this page is still in heavy construction, every guest is welcomed to left your trace on the "Doodle Board".
Our Team Project Parts Experiments Communication Links Doodle Board
Team Members
Luying Jia Yuanfan Yang Gechong Ruan Shan Lin Xin Rong Yicheng Long Ziying Liu Yuemeng Wang Yilong Zou Zi Wang He Yang Yongqiang Gou Chao Wang Junjie Luo Cong Liu
Our Schedule
Time Events Dec. 15, 2007 Team of Tsinghua University Founded Dec. 31, 2007 Brain Storm(1) Jan-Feb, 2008 Backgroud Investigation Feb.25, 2008 Brain Storm(2) Mar., 2008 Experimental procedure design; Modeling in silico Started Apr.1,2008 Wet Lab work started Apr.-Aug. ,2008 Wet Lab Work May.1, 2008 Team Registry May.25, 2008 Students’ Association of Synthetic Biology(SASB) Founded June.21, 2008 “Teach the teacher “workshop, in Kyoto, Japan Sept.-Oct., 2008 Inspection and Revision Oct.20,2008 Summary, Presentation Preparation Nov.8-9,2008 Jamboree In MIT,
Project
This year we are trying to work at the motility of bacteria. E.coli senses some chemicals and moves toward
or stays still, this behavior is called chemotaxis. Any modification of the pathway from the sensor to the
motile apparatus will cause into different chemotactic effcts.
Parts
Communication
Experiment and Protocol
1 PCR 1.1 Gold Faster Taq system: Reagent Concentration/Activity 50ul 100ul GF taq buffer 10x 5 10 GF taq 0.5 0.5 dNTPmix 10mM each 1 2 Primer 1 10uM 1 2 Primer 2 10um 1 2 Template DNA changeable 0.5 1 MgCl2 0.2M 0.5 1 ddH2O --- 40.5 81 1.2 The program under Gold faster taq system Progress Program I Program 2 Predenaturing 95℃ 2-5 min 95℃ 2-5 min Denaturing 95℃ 10-20sec 95℃ 10-20sec Annealing (Tm-5) ℃ 2-5 sec 68℃ 10-15sec/1kb Extension 72℃ 10-15sec/1kb
25-30cycle 25-30cycle
Last extension 1-2min or skipped 1-2min or skipped
2 Restriction cut: Reagent Concentration/Activity Volume(50ul system) Restriction cut buffer 10x 5ul Enzyme 1 -- 2.5ul Enzyme 2 -- 2.5ul Incubate at 37℃, 1.5 hrs or longer (Enzymes from Takara Co., Ltd)
3 Ligation: Reagent Volume(10ul system) Solution I 5ul DNA fragment 3.5ul Vector 1.5ul Incubate at 16-18℃,1hr or longer (Ligation kit from Takara)
Links
Tsinghua University: http://www.tsinghua.edu.cn/qhdwzy/index.jsp Parts: http://partsregistry.org Beijing Normal University: https://2008.igem.org/Team:Beijing_Normal ETH_Zurich: https://2008.igem.org/Team:ETH_Zurich Nature: http://www.nature.com Science: http://www.sciencemag.org System and Synthetic Biology: http://www.springer.com/biomed/journal/11693
Doodle Board
Oh, My E.coli, where are you! Yilong 08.7.11