Team:Tsinghua

From 2008.igem.org

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Although this page is still in heavy construction, every guest is welcomed to left your trace on the "Doodle Board".
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                      '''Internationally Genetically Engineered Machine Competition'''
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        [[[['''Main Page''']]]]      [Our Team]https://2008.igem.org/wiki/Team:Tsinghua/Our team
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      Main Page      Our Team      Project    Laboratory Work     Parts     Communication   Doodle Board
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      [ Project]        [[Parts]]
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            [[ Experiments]]          [[Communication]]    [[Links]]          [[Doodle Board]]
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Our  Team
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[[Image:Girls' Day 4.jpg]]
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==Team Members ==
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      Luying Jia  [[Image:Luying Jia.jpg]]                    Yuanfan Yang
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      Gechong Ruan                Shan Lin
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      Xin Rong                      Yicheng Long[[Image:Yicheng Long.jpg]]
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      Ziying Liu                      Yuemeng Wang [[Image:Yuemeng Wang.jpg]]
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      Yilong Zou [[Image:Yilong Zou.jpg]]                    Zi Wang[[Image:Zi Wang.jpg]]
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      He Yang[[Image:He Yang.jpg]]
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      Yongqiang Gou                Chao Wang[[Image:Chao Wang.jpg]]
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      Junjie Luo                    Cong Liu[[Image:Cong Liu.jpg]]
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Our Schedule
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        Time               Events
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  Dec. 15, 2007               Team of Tsinghua University Founded
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  Dec. 31, 2007               Brain Storm(1)
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  Jan-Feb, 2008               Backgroud Investigation
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  Feb.25, 2008               Brain Storm(2)
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  Mar., 2008               Experimental procedure design;
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                                Modeling in silico Started 
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  Apr.1,2008               Wet Lab work started
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  Apr.-Aug. ,2008       Wet Lab Work
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  May.1, 2008               Team Registry
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  May.25, 2008               Students’ Association of Synthetic Biology(SASB) Founded
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  June.21, 2008               “Teach the teacher “workshop, in Kyoto, Japan
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  Sept.-Oct., 2008       Inspection and Revision   
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  Oct.20,2008               Summary, Presentation Preparation
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  Nov.8-9,2008               Jamboree In MIT,
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Project
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  This year we are trying to work at the motility of bacteria. E.coli senses some chemicals and moves toward
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or stays still, this behavior is called chemotaxis. Any modification of the pathway from the sensor to the
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motile apparatus will cause into different chemotactic effcts.
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Parts
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Communication
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Experiment and Protocol
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     1  PCR
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  1.1 Gold Faster Taq system:
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     Reagent           Concentration/Activity 50ul 100ul
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     GF taq buffer        10x                         5      10
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    GF taq                                 0.5   0.5
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    dNTPmix             10mM each                 1   2
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    Primer 1             10uM                 1   2
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    Primer 2             10um                 1   2
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    Template DNA       changeable                 0.5   1
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    MgCl2             0.2M                 0.5   1
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    ddH2O                 ---                 40.5 81
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  1.2 The program under Gold faster taq system
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    Progress     Program I                 Program 2
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    Predenaturing   95℃        2-5 min         95℃  2-5 min
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    Denaturing     95℃        10-20sec         95℃  10-20sec
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    Annealing     (Tm-5) ℃  2-5 sec         68℃  10-15sec/1kb
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    Extension     72℃        10-15sec/1kb
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                  25-30cycle               25-30cycle
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    Last extension   1-2min or skipped       1-2min or skipped
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    2 Restriction cut:
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            Reagent         Concentration/Activity Volume(50ul system)
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            Restriction cut buffer 10x           5ul
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            Enzyme 1                 --           2.5ul
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            Enzyme 2                 --           2.5ul
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  Incubate at 37℃, 1.5 hrs or longer
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  (Enzymes from Takara Co., Ltd)
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      3  Ligation:
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              Reagent     Volume(10ul system)
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              Solution I 5ul
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              DNA fragment 3.5ul
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              Vector         1.5ul
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    Incubate at 16-18℃,1hr or longer
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    (Ligation kit from Takara)
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Links
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  Tsinghua University:
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      http://www.tsinghua.edu.cn/qhdwzy/index.jsp
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  Parts:
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      http://partsregistry.org
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  Beijing Normal University:
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      https://2008.igem.org/Team:Beijing_Normal
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  ETH_Zurich:
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      https://2008.igem.org/Team:ETH_Zurich
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  Nature:
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      http://www.nature.com
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  Science:
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      http://www.sciencemag.org
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  System and Synthetic Biology:
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      http://www.springer.com/biomed/journal/11693
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Doodle Board
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  Oh, My E.coli, where are you!        Yilong 08.7.11
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Revision as of 17:43, 30 July 2008

                     Internationally Genetically Engineered Machine Competition

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Retrieved from "http://2008.igem.org/Team:Tsinghua"