Team:Tsinghua
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== Introduction == | == Introduction == | ||
- | Motile bacteria can respond to chemical gradients in their environment. Such directed movement in response to special chemicals is defined as chemotaxis. If organisms detect substances beneficial for surviving, positive chemotaxis occurs and cells will move in the direction of increasing concentration. In contrast, negative chemotaxis occurs when motile bacteria move away from a repellant. Both kinds of responses adjust the behaviors of organisms to the environment, thus improve their ability of surviving. | + | Motile bacteria can respond to chemical gradients in their environment. Such directed movement in response to special chemicals is defined as chemotaxis. |
+ | If organisms detect substances beneficial for surviving, positive chemotaxis occurs and cells will move in the direction of increasing concentration. In contrast, negative chemotaxis occurs when motile bacteria move away from a repellant. Both kinds of responses adjust the behaviors of organisms to the environment, thus improve their ability of surviving. | ||
One typical chemotactic behavior is the response of Escherichia coli toward certain nutritions, which plays a key role in seeking food. Researchers have investigated thoroughly into the molecular mechanism of it. | One typical chemotactic behavior is the response of Escherichia coli toward certain nutritions, which plays a key role in seeking food. Researchers have investigated thoroughly into the molecular mechanism of it. | ||
Motility in prokaryotes is accomplished by flagellum, a complex structure moving the cell by rotation, much like the propeller in a motor boat. In E.coli, counterclockwise rotation moves the cell in a direction called a run, while clockwise rotation results in tumbling of the cell. | Motility in prokaryotes is accomplished by flagellum, a complex structure moving the cell by rotation, much like the propeller in a motor boat. In E.coli, counterclockwise rotation moves the cell in a direction called a run, while clockwise rotation results in tumbling of the cell. |
Revision as of 18:37, 30 July 2008
Internationally Genetically Engineered Machine Competition
Main Page Our Team Project Modeling Laboratory Work Parts Communication Doodle Board
== Team Roster ==
Guoqiang Chen Professor chengq@mail.tsinghua.edu.cn Shao Li Professor shaoli@tsinghua.edu.cn Zhirong Sun Professor sunzhr@mail.tsinghua.edu.cn
Syna Gao Ph.D synagao@gmail.com Tao Ma Ph.D mat07@mails.thu.edu.cn Yanchao Han Ph.D haytcb@gmail.com Feng Liu Undergraduate liufeng04@mails.tsinghua.edu.cn
Junjie Luo Undergraduate jjluo54@163.com Yaxuan Yang Undergraduate yangyx04@mails.tsinghua.edu.cn Jun Wang Undergraduate wj-cn@163.com Chao Wang Undergraduate wangchao05@mails.tsinghua.edu.cn Qi Liu Undergraduate q-liu05@mails.tsinghua.edu.cn Shiqing Wang Undergraduate wangsq05@mails.thu.edu.cn Tong Si Undergraduate sit05@mails.thu.edu.cn Shan Lin Undergraduate coolls03@163.com Yilong Zou Undergraduate ylzouyilong@yahoo.cn Ziying Liu Undergraduate liuzy05@mails.tsinghua.edu.cn Xing Rong Undergraduate rongx05@mails.thu.edu.cn Yicheng Long Undergraduate longyc05@mails.tsinghua.edu.cn Luying Jia Undergraduate jialy05@mails.tsinghua.edu.cn Yuemeng Wang Undergraduate wym05@mails.tsinghua.edu.cn Junya Peng Undergraduate junya.peng@gmail.com Xiaoxi Yuan Undergraduate tangbf1983@gmail.com Chong Wang Undergraduate wangchongxn@yahoo.com.cn Feng Tian Undergraduate tianf06@mails.tsinghua.edu.cn Yuanfan Yang Undergraduate yangyf06@mails.tsinghua.edu.cn Liang Zheng Undergraduate 1000zhengliang@sina.com Yongqiang Gou Undergraduate gouyongqiang007@163.com He Yang Undergraduate yanghemary@gmail.com Zi Wang Undergraduate fiona99988@yahoo.com.cn Gechong Ruan Undergraduate rgc06@mails.tsinghua.edu.cn Bing Xing Undergraduate binxing1987@gmail.com
== Team Members == Junjie Luo Yaxuan Yan Jun Wang
Chao Wang Qi Liu Shiqing Wang Tong Si
Shan Lin Yilong Zou Ziying Liu Xin Rong Yicheng Long Luying Jia Yuemeng Wang Junya Peng
Xiaoxi Yuan Chong Wang Feng Tian Yuanfan Yang
Liang Zheng Yongqiang Gou He Yang
Zi Wang Gechong Ruan Bing Xing
== Our Schedule ==
== Introduction == Motile bacteria can respond to chemical gradients in their environment. Such directed movement in response to special chemicals is defined as chemotaxis.
If organisms detect substances beneficial for surviving, positive chemotaxis occurs and cells will move in the direction of increasing concentration. In contrast, negative chemotaxis occurs when motile bacteria move away from a repellant. Both kinds of responses adjust the behaviors of organisms to the environment, thus improve their ability of surviving. One typical chemotactic behavior is the response of Escherichia coli toward certain nutritions, which plays a key role in seeking food. Researchers have investigated thoroughly into the molecular mechanism of it. Motility in prokaryotes is accomplished by flagellum, a complex structure moving the cell by rotation, much like the propeller in a motor boat. In E.coli, counterclockwise rotation moves the cell in a direction called a run, while clockwise rotation results in tumbling of the cell. The direction of rotation is controlled by phosphorylation of CheY, a signaling protein in the chemotaxis pathway of E.coli. Low level of phosphorylation results in counterclockwise rotation of flagella and makes the cell move forward. In contrast, phosphorylated CheY favors tumbling. The level of protein phosphorylation is regulated by certain attractant or repellent, which can bind specific receptors on the cell surface. Signals are then passed through a series of intracellular biochemical reactions, in which phosphorylation of CheY is controlled. In this way bacteria accomplish chemotactic behavior.
Escherichia coli is a common kind of engineer bacteria, which is convenient for genetic manipulation. It is likely that we make use of the chemotactic pathway in E.coli, making them detect and move toward a special chemical substance, maybe a certain kind of pollutant. If the genetic engineering strain also has the ability of degrading aimed chemicals, efficiency of pollution treatment can be hopefully improved a lot. However, the natural ligands concerning chemotaxis in E.coli are limited in several kinds of nutrition, such as carbohydrate and amino acids. Strains that can detecting more kinds of organic substances and even heavy metal ions is planed to be got through the reconstruction of chemotaxis system.
== Background ==
== Design ==
== Predicted Result ==
== Related literature ==
== Scheme ==
== Lab Log ==
== Protocols ==
== Molecular cloning ==
== Bacteria behavior ==
[[Devices and Parts
Parts]]
== Protein Coding ==
== Regulatory ==
== Vectors ==
== Others ==
Devices
== Meet up ==
== Phone ==
== E-mails ==
Contact us: sasbthu@yahoo.cn