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Team:Tsinghua
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Our Team Project Parts Experiments Communication Links Doodle Board
Our Team
Luying JiaYuanfan Yang Gechong Ruan Shan Lin Xin Rong Yicheng Long
Ziying Liu Yuemeng Wang
Yilong Zou
Zi Wang
He Yang
Yongqiang Gou Chao Wang
Junjie Luo Cong Liu
Project
This year we are trying to work at the motility of bacteria. E.coli senses some chemicals and moves toward or stays still, this behavior is called chemotaxis. Any modification of the pathway from the sensor to the motile apparatus will cause into different chemotactic effcts.
Parts
Communication
Experiments
PCR Gold Faster Taq system: Reagent Concentration/Activity 50ul 100ul
GF taq buffer 10x 5 10
GF taq 0.5 0.5
dNTPmix 10mM each 1 2
Primer 1 10uM 1 2
Primer 2 10um 1 2
Template DNA changeable 0.5 1
MgCl2 0.2M 0.5 1
ddH2O --- 40.5 81
Progress Program I Program 2 Predenaturing 95℃ 2-5 min 95℃ 2-5 min
Denaturing 95℃ 10-20sec 95℃ 10-20sec Annealing (Tm-5) ℃ 2-5 sec 68℃ 10-15sec/1kb Extension 72℃ 10-15sec/1kb 25-30cycle 25-30cycle Last extension 1-2min or skipped 1-2min or skipped
Links
Tsinghua University: http://www.tsinghua.edu.cn/qhdwzy/index.jsp
Parts: http://partsregistry.org
iGEM Headquarter: hq@igem.org
Beijing Normal University:https://2008.igem.org/Team:Beijing_Normal
ETH_Zurich:https://2008.igem.org/Team:ETH_Zurich
Nature:http://www.nature.com
Science:http://www.sciencemag.org
System and Synthetic Biology:http://www.springer.com/biomed/journal/11693
Doodle Board
Oh, My E.coli, where are you! Yilong 08.7.11
