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Team:Tsinghua
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[[[[Main Page]]]] Our Team Project Parts Experiments Communication Links Doodle Board
Our Team
Team Members
Luying JiaYuanfan Yang Gechong Ruan Shan Lin Xin Rong Yicheng Long
Ziying Liu Yuemeng Wang
Yilong Zou
Zi Wang
He Yang
Yongqiang Gou Chao Wang
Junjie Luo Cong Liu
Our Schedule
Time Events
Dec. 15, 2007 Team of Tsinghua University Founded
Dec. 31, 2007 Brain Storm(1)
Jan-Feb, 2008 Backgroud Investigation
Feb.25, 2008 Brain Storm(2)
Mar., 2008 Experimental procedure design;
Modeling in silico Started
Apr.1,2008 Wet Lab work started
Apr.-Aug. ,2008 Wet Lab Work
May.1, 2008 Team Registry
May.25, 2008 Students’ Association of Synthetic Biology(SASB) Founded
June.21, 2008 “Teach the teacher “workshop, in Kyoto, Japan
Sept.-Oct., 2008 Inspection and Revision
Oct.20,2008 Summary, Presentation Preparation
Nov.8-9,2008 Jamboree In MIT,
Project
This year we are trying to work at the motility of bacteria. E.coli senses some chemicals and moves toward
or stays still, this behavior is called chemotaxis. Any modification of the pathway from the sensor to the
motile apparatus will cause into different chemotactic effcts.
Parts
Communication
Experiment and Protocol
1 PCR
1.1 Gold Faster Taq system:
Reagent Concentration/Activity 50ul 100ul
GF taq buffer 10x 5 10
GF taq 0.5 0.5
dNTPmix 10mM each 1 2
Primer 1 10uM 1 2
Primer 2 10um 1 2
Template DNA changeable 0.5 1
MgCl2 0.2M 0.5 1
ddH2O --- 40.5 81
1.2 The program under Gold faster taq system
Progress Program I Program 2
Predenaturing 95℃ 2-5 min 95℃ 2-5 min
Denaturing 95℃ 10-20sec 95℃ 10-20sec
Annealing (Tm-5) ℃ 2-5 sec 68℃ 10-15sec/1kb
Extension 72℃ 10-15sec/1kb
25-30cycle 25-30cycle
Last extension 1-2min or skipped 1-2min or skipped
2 Restriction cut:
Reagent Concentration/Activity Volume(50ul system)
Restriction cut buffer 10x 5ul
Enzyme 1 -- 2.5ul
Enzyme 2 -- 2.5ul
Incubate at 37℃, 1.5 hrs or longer
(Enzymes from Takara Co., Ltd)
3 Ligation:
Reagent Volume(10ul system)
Solution I 5ul
DNA fragment 3.5ul
Vector 1.5ul
Incubate at 16-18℃,1hr or longer
(Ligation kit from Takara)
Links
Tsinghua University:
http://www.tsinghua.edu.cn/qhdwzy/index.jsp
Parts:
http://partsregistry.org
Beijing Normal University:
https://2008.igem.org/Team:Beijing_Normal
ETH_Zurich:
https://2008.igem.org/Team:ETH_Zurich
Nature:
http://www.nature.com
Science:
http://www.sciencemag.org
System and Synthetic Biology:
http://www.springer.com/biomed/journal/11693
Doodle Board
Oh, My E.coli, where are you! Yilong 08.7.11
