"
Team:Tsinghua
From 2008.igem.org
| HOME | Team | Project | Parts | Modelling | Notebook | Doodle Board |
|---|
Contents |
Intro of Our Project
| Tsinghua University was established in 1911, originally under the name “Tsinghua Xuetang”. The school was renamed "Tsinghua School" in 1912. The university section was founded in 1925. The name “National Tsinghua University” was adopted in 1928.click here to see more pictures
The educational philosophy of Tsinghua is to "train students with integrity." Among over 120,000 students who have graduated from Tsinghua since its founding are many outstanding scholars, eminent entrepreneurs and great statesmen remembered and respected by their fellow Chinese citizens. With the motto of “Self-Discipline and Social Commitment” and the spirit of “Actions Speak Louder than Words”, Tsinghua University is dedicated to the well-being of Chinese society and to world development. |  |
News
GF taq is recommended in our PCR reactions. 2008.June.25
The first plasmid is successfully constrcuted. 2008.Jul.20
The gene we need is at synthesis in Geneart. 2008.Jul.24
The standard vector pSB1A3 lost in the transformation. 2008.Jul.28
Then it finally succeeds. 2008.Jul.31
Template of one of the receptors are still in vacancy.
New edition of project interpretation are in process.
Intro of Tsinghua University
| Tsinghua University was established in 1911, originally under the name “Tsinghua Xuetang”. The school was renamed "Tsinghua School" in 1912. The university section was founded in 1925. The name “National Tsinghua University” was adopted in 1928.click here to see more pictures
The educational philosophy of Tsinghua is to "train students with integrity." Among over 120,000 students who have graduated from Tsinghua since its founding are many outstanding scholars, eminent entrepreneurs and great statesmen remembered and respected by their fellow Chinese citizens. With the motto of “Self-Discipline and Social Commitment” and the spirit of “Actions Speak Louder than Words”, Tsinghua University is dedicated to the well-being of Chinese society and to world development. |  |
== Our Schedule ==
Time Events
Dec. 15, 2007 Team of Tsinghua University Founded
Dec. 31, 2007 Brain Storm(1)
Jan-Feb, 2008 Backgroud Investigation
Feb.25, 2008 Brain Storm(2)
Mar., 2008 Experimental procedure design;
Modeling in silico Started
Apr.1,2008 Wet Lab work started
Apr.-Aug.,2008 Wet Lab Work
May.1, 2008 Team Registry
May.25, 2008 Students’ Association of Synthetic Biology(SASB) Founded
June.21, 2008 “Teach the teacher “workshop, in Kyoto, Japan
Sept.-Oct., 2008 Inspection and Revision
Oct.20,2008 Summary, Presentation Preparation
Nov.8-9,2008 Jamboree In MIT
Molecular cloning
1 PCR
1.1 Gold Faster Taq system:
Reagent Concentration/Activity 50ul 100ul
GF taq buffer 10x 5 10
GF taq 0.5 0.5
dNTPmix 10mM each 1 2
Primer 1 10uM 1 2
Primer 2 10um 1 2
Template DNA changeable 0.5 1
MgCl2 0.2M 0.5 1
ddH2O --- 40.5 81
1.2 The program under Gold faster taq system
Progress Program I Program 2
Predenaturing 95℃ 2-5 min 95℃ 2-5 min
Denaturing 95℃ 10-20sec 95℃ 10-20sec
Annealing (Tm-5) ℃ 2-5 sec 68℃ 10-15sec/1kb
Extension 72℃ 10-15sec/1kb
25-30cycle 25-30cycle
Last extension 1-2min or skipped 1-2min or skipped
2 Restriction cut:
Reagent Concentration/Activity Volume(50ul system)
Restriction cut buffer 10x 5ul
Enzyme 1 -- 2.5ul
Enzyme 2 -- 2.5ul
Incubate at 37℃, 1.5 hrs or longer
(Enzymes from Takara Co., Ltd)
3 Ligation:
Reagent Volume(10ul system)
Solution I 5ul
DNA fragment 3.5ul
Vector 1.5ul
Incubate at 16-18℃,1hr or longer
(Ligation kit from Takara)
| Home | Team | Project | Parts | Modelling | Notebook | Doodle Board |
|---|
