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Team:UCSF
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<h2 align="left">Epigenetic control of gene expression</h2> | <h2 align="left">Epigenetic control of gene expression</h2> | ||
<p align="left">The cells of higher eukaryotes utilize chromatin state to encode "permanent" epigenetic changes in gene expression. For example, signals received by a cell during the course of development can induce the partitioning of the genome into accessible (euchromatin) and inaccessible (heterochromatin) regions that specify the fate of that cell. This epigenetic profile, in which blocks of gene are "silenced" by heterochromatin, is stably maintained and inherited by daughter cells. Thus, chromatin state provides a higher level of gene expression control that is regional (acting on many genes at once), dominant over transcription factors, ultra-cooperative (all or none), and highly stable (memory). Engineerable control over chromatin state would clearly be a powerful tool for Synthetic Biology. We have constructed and characterized a synthetic silencing system in <em>S. cerevisiae</em> in which we can inducibly silence specific loci in the genome. This foundational technology will facilitate the construction of complex genetic circuits with memory, and has potential application in the engineering of cell differentiation in higher eukaryotes.</p> | <p align="left">The cells of higher eukaryotes utilize chromatin state to encode "permanent" epigenetic changes in gene expression. For example, signals received by a cell during the course of development can induce the partitioning of the genome into accessible (euchromatin) and inaccessible (heterochromatin) regions that specify the fate of that cell. This epigenetic profile, in which blocks of gene are "silenced" by heterochromatin, is stably maintained and inherited by daughter cells. Thus, chromatin state provides a higher level of gene expression control that is regional (acting on many genes at once), dominant over transcription factors, ultra-cooperative (all or none), and highly stable (memory). Engineerable control over chromatin state would clearly be a powerful tool for Synthetic Biology. We have constructed and characterized a synthetic silencing system in <em>S. cerevisiae</em> in which we can inducibly silence specific loci in the genome. This foundational technology will facilitate the construction of complex genetic circuits with memory, and has potential application in the engineering of cell differentiation in higher eukaryotes.</p> | ||
| - | <p align="right"><a href=" | + | <p align="right"><a href="https://2008.igem.org/Team:UCSF/Project">More...</a></p> |
<p align="right"> </p> | <p align="right"> </p> | ||
<table width="870" border="0" cellpadding="3"> | <table width="870" border="0" cellpadding="3"> | ||
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<h3>OUR TEAM</h3> | <h3>OUR TEAM</h3> | ||
<blockquote> | <blockquote> | ||
| - | <h4>Team members</h4> | + | <h4><a href:"https://2008.igem.org/Team:UCSF/Team">Team members</a></h4> |
<h4>Individual Contributions</h4> | <h4>Individual Contributions</h4> | ||
<h4>Notebooks</h4> | <h4>Notebooks</h4> | ||
Revision as of 18:22, 24 October 2008
CHROMATIN MEMORIES
Epigenetic control of gene expression
The cells of higher eukaryotes utilize chromatin state to encode "permanent" epigenetic changes in gene expression. For example, signals received by a cell during the course of development can induce the partitioning of the genome into accessible (euchromatin) and inaccessible (heterochromatin) regions that specify the fate of that cell. This epigenetic profile, in which blocks of gene are "silenced" by heterochromatin, is stably maintained and inherited by daughter cells. Thus, chromatin state provides a higher level of gene expression control that is regional (acting on many genes at once), dominant over transcription factors, ultra-cooperative (all or none), and highly stable (memory). Engineerable control over chromatin state would clearly be a powerful tool for Synthetic Biology. We have constructed and characterized a synthetic silencing system in S. cerevisiae in which we can inducibly silence specific loci in the genome. This foundational technology will facilitate the construction of complex genetic circuits with memory, and has potential application in the engineering of cell differentiation in higher eukaryotes.
OUR PROJECT
Project Summary
Synthetic Chromatin Bit
- Design
- Properties
- Higher-Order Systems
- Modeling
- FAQs
OUR TEAM
Team members
Individual Contributions
Notebooks
Resources
FAQs
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Human Practices | Notebooks |
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