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Team:UNIPV-Pavia/Notebook/Week2
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Notebook
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Week 2: 05/26/08 - 05/29/08
05/27/08
- Team meeting at Biomedical Informatics Lab to talk about UNIPV-Pavia team presentation at Paris Teacher's Workshop and to make our first wiki updating.
- We received restriction enzymes and Agarose Gel DNA Extraction Kit from Roche. Now we are ready to cut;)
05/28/08
- We performed plasmid digestion for 9 parts (1 µg):
| BBa_C0078 (X-P) | BBa_E0040 (X-P) | BBa_E1010 (X-P) | BBa_C0051 (X-P) |
| BBa_B0030 (X-P) | BBa_B0030 (E-X) | BBa_B0030 (S-P) | BBa_E0240 (E-X) |
| BBa_J23100 (E-S) |
- We had to insulate excided fragments for:
| BBa_C0078 (X-P) | BBa_E0040 (X-P) | BBa_E1010 (X-P) | BBa_C0051 (X-P) |
| BBa_B0030 (X-P) | BBa_J23100 (E-S) |
- While we had to insulate opened plasmids for:
| BBa_B0030 (E-X) | BBa_B0030 (S-P) | BBa_E0240 (E-X) |
- We ran two different gels for these two groups, because excided fragments are smaller then opened plasmids.
- Unfortunately, BBa_B0030 (X-P) and BBa_J23100 (E-S) fragments had smearing appearance because they were smaller then other excided fragments and ran too fast.
- We performed gel extraction for all the 9 parts, but we decided to repeat cutting/run/extraction process for BBa_B0030 (X-P) and BBa_J23100 (E-S) in the next days, to perform a more efficient extraction for these 2 parts.
- We took 15 µl from BBa_B0030 and BBa_J23100 stocks and infected 9 ml LB + Amp for each part. We incubated the 9 ml cultures at 37°C overnight. (We already had at our disposal extracted plasmids for these 2 parts, but we performed plasmid extraction anyway to have more).
05/29/08
