Team:UNIPV-Pavia/Notebook/Week3
From 2008.igem.org
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- | *We transformed 60 the 6 parts we received from IGEM HQ: | + | *We transformed 60 µl of TOP10 with 2 µl of the 6 parts (DNA + glycerol) we received from IGEM HQ: |
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|BBa_I15010 | |BBa_I15010 | ||
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+ | *We noticed that IGEM 2007 teams which used luxI and lasR parts choosed BBa_C0061 instead of BBa_C0161 and BBa_C0079 instead of BBa_C0179. So, we decided in addition to amplify BBa_C0061 and BBa_C0079; we shall see later which luxI and lasR to choose. | ||
+ | |||
+ | *So, we cut |
Revision as of 11:00, 7 June 2008
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Notebook
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Week 3: 06/03/08 - 06/06/08
06/03/08
- We transformed 60 µl of TOP10 with 2 µl of the 6 parts (DNA + glycerol) we received from IGEM HQ:
BBa_C0179 | BBa_C0161 | BBa_R0051 | BBa_I14032 |
BBa_I15008 | BBa_I15010 |
- We noticed that IGEM 2007 teams which used luxI and lasR parts choosed BBa_C0061 instead of BBa_C0161 and BBa_C0079 instead of BBa_C0179. So, we decided in addition to amplify BBa_C0061 and BBa_C0079; we shall see later which luxI and lasR to choose.
- So, we cut