"
Team:UNIPV-Pavia/Notebook/Week3
From 2008.igem.org
(Difference between revisions)
| Line 82: | Line 82: | ||
|} | |} | ||
| - | * | + | *We repeated tra |
| + | |||
| + | *We picked up one colony from BBa_C0061, BBa_R0051, BBa_I14032, I15008 and I15010 plates to grow 5 ml cultures of transformed bacteria overnight. | ||
Revision as of 11:37, 7 June 2008
 Home
|  The Team
|  The Project
|  Biological Safety
|  Parts Submitted to the Registry
|
|---|---|---|---|---|
 Dry Lab
|  Wet Lab
|  Modeling
|  Protocols
|  Activity Notebook
|
Notebook
| Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 |
|---|
Week 3: 06/03/08 - 06/06/08
06/03/08
- We transformed 60 µl of TOP10 with 2 µl of the 6 parts (DNA + glycerol) we received from IGEM HQ:
| BBa_C0179 | BBa_C0161 | BBa_R0051 | BBa_I14032 |
| BBa_I15008 | BBa_I15010 |
- NOTE: We noticed that IGEM 2007 teams which used luxI or lasR parts choosed BBa_C0061 instead of BBa_C0161 and BBa_C0079 instead of BBa_C0179. So, we decided in addition to amplify BBa_C0061 and BBa_C0079; we shall see later which luxI and lasR to choose.
- So, we cut paper spots for BBa_C0061 and BBa_C0079 and resuspended them in 10 µl of warmed TE buffer.
- We transformed these 2 parts using 4 µl of DNA in TE.
- We plated transformed bacteria and incubated them at 37°C overnight.
06/04/08
- After overnight incubation, the following 5 plates showed colonies:
| BBa_C0061 | BBa_R0051 |
| BBa_I14032 | BBa_I15008 |
| BBa_I15010 |
- while the following 3 plates did not:
| BBa_C0079 | BBa_C0179 |
| BBa_C0161 |
- We repeated tra
- We picked up one colony from BBa_C0061, BBa_R0051, BBa_I14032, I15008 and I15010 plates to grow 5 ml cultures of transformed bacteria overnight.
