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| - | <P ALIGN=CENTER STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=7 STYLE="font-size: 54pt"><B>The
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| - | Chimeras</B></FONT></FONT></FONT></P>
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| - | <P ALIGN=CENTER STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=7 STYLE="font-size: 54pt"><B><FONT SIZE=5 STYLE="font-size: 20pt">(Blurring
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| - | the line between plant and bacteria)</FONT> </B></FONT></FONT></FONT>
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| - | <P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>A
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| - | Brief Introduction</B></FONT></FONT></FONT></P>
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| - | <P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>Our
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| - | project focuses on creating the very first synthetic biology plant
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| - | chassis ever presented as a IGEM project. We plan on doing this by
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| - | taking advantage of already existing photosensors and temperature
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| - | switches existing in plants. Our end result will be photo and
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| - | temperature sensitive grass. The idea would be to introduce two
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| - | different lux operons encoding for two different colors and have the
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| - | expression of them controlled by light and temperature. During the
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| - | day both lux pathways would be turned off by the photosensor switch,
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| - | at night the one of the lux operons would be turned on decided by the
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| - | temperature switch.</B></FONT></FONT></FONT></P>
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| - | <P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>The
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| - | Photo Sensor</B></FONT></FONT></FONT></P>
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| - | <P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>Our
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| - | photo sensor would take advantage of an already existing red/far red
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| - | pathway existing in plants. Pathway is normally used to detect
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| - | shading by other plants as well as the end of the day. When red light
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| - | is present the PR protein turns into PFR which activates another
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| - | protein which then binds to LRE (Light Responsive Element) section of
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| - | the DNA this then activates the production of rubisco. When the plant
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| - | is exposed to far red light (such as would occur at the end of the
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| - | day) the PFR protien converts back to PR and the pathway shuts off.
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| - | Our project will take advantage of this pathway and create an LRE
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| - | biobrick which can then be used as a photosensor. In our project LRE
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| - | will be attached to a luxR to allow for the lux pathway to be shut
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| - | off during the day and turn on during the night. </B></FONT></FONT></FONT>
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| - | </P>
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| - | <P ALIGN=LEFT STYLE="background: #000000"><BR><BR>
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| - | </P>
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| - | <P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>The
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| - | Temperature Sensor</B></FONT></FONT></FONT></P>
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| - | <P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>The
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| - | temperature sensor will be cold sensitive that will cause the
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| - | production of a repressor which will be used to repress one of the
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| - | pathways (lux2). The repressed lux2 pathway carries another repressor
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| - | for the lux1 pathway that is transcribed only when the temperature
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| - | senor is turned off. This will allow for the change of color due to
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| - | temperature. </B></FONT></FONT></FONT>
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| - | <P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>Our
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| - | Team </B></FONT></FONT></FONT> | + | |
| - | </P>
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| - | <P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>Full
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| - | Time</B></FONT></FONT></FONT></P>
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| - | <P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>Part
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| - | Time </B></FONT></FONT></FONT>
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| - | </P>
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| - | </P>
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| - | <P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>The
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| - | Lab Book</B></FONT></FONT></FONT></P>
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| - | </P>
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| - | <P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>Our
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| - | Sponsors</B></FONT></FONT></FONT></P>
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| - | <P ALIGN=LEFT STYLE="background: #000000"><FONT COLOR="#00ff00"><FONT FACE="Estrangelo Nisibin Outline"><FONT SIZE=4 STYLE="font-size: 15pt"><B>Special
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| - | Thanks </B></FONT></FONT></FONT>
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