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Team:University of Lethbridge/Notebook/Project1June
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Plasma mini prepped pTopp cells | Plasma mini prepped pTopp cells | ||
| - | Attempted to make RP1616 cells competent using [http://openwetware.org/wiki/Preparing_chemically_competent_cells]. 100 uL aliquots of cells were froxen in liquid nitrogen and stored in the -80 freezer. | + | Attempted to make RP1616 cells competent using the open wet ware [http://openwetware.org/wiki/Preparing_chemically_competent_cells protocol]. 100 uL aliquots of cells were froxen in liquid nitrogen and stored in the -80 freezer. |
Ran CheZ in pUC19 plasmid and pUC19 plasmid from last year | Ran CheZ in pUC19 plasmid and pUC19 plasmid from last year | ||
| - | [[Image:puc19 + cheZ.jpg]] | + | [[Image:puc19 + cheZ.jpg|500 px]] |
Revision as of 01:13, 3 July 2008
Contents |
June 16, 2008
Nathan Puhl, Munima, Christa, Sebastian, Roxanne
Plated cheZ knockout strain from glycerol on LB + amp and Plain LB to assess viability and antibiotic resistance at 37 C overnight.
June 17 2008
Munima, Christa, Nathan Puhl
cheZ knockout strain viable on LB, no growth on LB + amp - Good
June 30, 2008
Nathan Puhl, Christa, Munima
plated RP1616 and pTopp cells from glycerol stocks onto LB and LB + amp, respectively
July 1, 2008
Nathan Puhl
Subcultured RP1616 into liquid LB and pTopp cells into liquid LB + amp
July 2, 2008
Nathan Puhl, Christa, Munima
Plasma mini prepped pTopp cells
Attempted to make RP1616 cells competent using the open wet ware [http://openwetware.org/wiki/Preparing_chemically_competent_cells protocol]. 100 uL aliquots of cells were froxen in liquid nitrogen and stored in the -80 freezer.
Ran CheZ in pUC19 plasmid and pUC19 plasmid from last year
