Team:University of Lethbridge/Notebook/Project1October

From 2008.igem.org

(Difference between revisions)
(October 6, 2008)
(Munima, John, Roxanne)
Line 56: Line 56:
  |<!--col1-->[[Image:Motility media - K12.jpg|280px]]
  |<!--col1-->[[Image:Motility media - K12.jpg|280px]]
  |<!--col2-->[[Image:Motility media - Staph.jpg|280px]]
  |<!--col2-->[[Image:Motility media - Staph.jpg|280px]]
-
 
  |-
  |-

Revision as of 03:35, 8 October 2008

Back to The University of Lethbridge Main Notebook

Contents

October 2, 2008

Munima, Christa, John

Objective: To set up chemotaxis spatial localization assay

Made and autoclaved media to make LB + amp plates to be used in the assay.

Recipe:

- Peptone 5g
- Yeast Extract 2.5 g
- NaCl 5 g 
- Agar (0.25 %) 1.25 g
- dH2O 500 mL


October 4, 2008

Christa

Objective: To set up chemotaxis spatail locatization assay

Media made on October 2 looked funny.

Poured 23 plates (LB + amp 100ug/mL) anyway. Stored in iGEM 4 C fridge.


October 6, 2008

Christa, Alix

Objective: Make more solutions for future chemical competency experiments.

Made 500 mL of 100 mM CaCl2 solution and 500 mL of 80mM MgCl2-20mM CaCl2 solution. They are stored on the counter under the iGEM glass cabinet in 1 L media bottles.

Selina

Objective: Create motility media stab tubes controls for motility assay.

Inoculated two 5 mL LB media tubes, one with E. coli K12 (wild-type), the other with Staphylococcus epidermis and let incubate at 37 C for ~8 hours.

Stabbed motility media (no theophylline) with subcultures of either cell type, using inoculating loops (teeny loop part, essentially a rod).

-Positive control: E. coli K12 (wild-type)
-Negative control: Staphylococcus epidermis

Incubated for 48 hours at 37 C.

Selina

Streaked the "LB+ amp chemically 'competent' cells transformed with pUC19 control plasmid" plate (Aug. 26) with wild-type E. coli to test for ampicillin presence.

Incubated overnight at 37 C.

October 7, 2008

Munima, John, Roxanne

Objective: Assess the motility media stab tubes after one day of incubation.

Motility media - K12.jpg Motility media - Staph.jpg
Positive control: K12 Negative control: Staphylococcus epidermis


Side-by-side comparison:

Motility media controls.jpg