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Team:University of Lethbridge/Notebook/Project1October
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October 2, 2008
Munima, Christa, John
Objective: To set up chemotaxis spatial localization assay
Made and autoclaved media to make LB + amp plates to be used in the assay.
Recipe:
- Peptone 5g - Yeast Extract 2.5 g - NaCl 5 g - Agar (0.25 %) 1.25 g - dH2O 500 mL
October 4, 2008
Christa
Objective: To set up chemotaxis spatail locatization assay
Media made on October 2 looked funny.
Poured 23 plates (LB + amp 100ug/mL) anyway. Stored in iGEM 4 C fridge.
October 6, 2008
Christa, Alix
Objective: Make more solutions for future chemical competency experiments.
Made 500 mL of 100 mM CaCl2 solution and 500 mL of 80mM MgCl2-20mM CaCl2 solution. They are stored on the counter under the iGEM glass cabinet in 1 L media bottles.
