Team:University of Lethbridge/Notebook/Project1October

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Contents

October 2, 2008

Munima, Christa, John

Objective: To set up chemotaxis spatial localization assay

Made and autoclaved media to make LB + amp plates to be used in the assay.

Recipe:

- Peptone 5g
- Yeast Extract 2.5 g
- NaCl 5 g 
- Agar (0.25 %) 1.25 g
- dH2O 500 mL


October 4, 2008

Christa

Objective: To set up chemotaxis spatail locatization assay

Media made on October 2 looked funny.

Poured 23 plates (LB + amp 100ug/mL) anyway. Stored in iGEM 4 C fridge.


October 6, 2008

Christa, Alix

Objective: Make more solutions for future chemical competency experiments.

Made 500 mL of 100 mM CaCl2 solution and 500 mL of 80mM MgCl2-20mM CaCl2 solution. They are stored on the counter under the iGEM glass cabinet in 1 L media bottles.

Selina

Objective: Retry the motility media stab tubes with the controls from the bio labs.

Positive control: K12 (parent strain of RP1616) Negative control: Staphylococcus epidermis

Regular motility media (no theophylline) was stabbed with either cell type from glycerol stocks, using inoculating loops.

Left in incubator overnight at 37 C. Should check results in 48 hours.


October 7, 2008

Munima, John, Roxanne

Objective: Assess the motility media stab tubes after one day of incubation.

Positive control: K12

Motility media - K12.jpg


Negative control: Staphylococcus epidermis

Motility media - Staph.jpg


Side-by-side comparison:

Motility media controls.jpg