Team:University of Lethbridge/Notebook/Project1October

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Contents

October 2, 2008

Munima, Christa, John

Objective: To set up chemotaxis spatial localization assay

Made and autoclaved media to make LB + amp plates to be used in the assay.

Recipe:

- Peptone 5g
- Yeast Extract 2.5 g
- NaCl 5 g 
- Agar (0.25 %) 1.25 g
- dH2O 500 mL


October 4, 2008

Christa

Objective: To set up chemotaxis spatail locatization assay

Media made on October 2 looked funny.

Poured 23 plates (LB + amp 100ug/mL) anyway. Stored in iGEM 4 C fridge.


October 6, 2008

Christa, Alix

Objective: Make more solutions for future chemical competency experiments.

Made 500 mL of 100 mM CaCl2 solution and 500 mL of 80mM MgCl2-20mM CaCl2 solution. They are stored on the counter under the iGEM glass cabinet in 1 L media bottles.

Selina

Objective: Create motility media stab tubes controls for motility assay.

Inoculated two 5 mL LB media tubes, one with E. coli K12 (wild-type), the other with Staphylococcus epidermis and let incubate at 37 C for ~8 hours.

Stabbed motility media (no theophylline) with subcultures of either cell type, using inoculating loops (teeny loop part, essentially a rod).

-Positive control: E. coli K12 (wild-type)
-Negative control: Staphylococcus epidermis

Incubated for 48 hours at 37 C.

October 7, 2008

Munima, John, Roxanne

Objective: Assess the motility media stab tubes after one day of incubation.

Motility media - K12.jpg Motility media - Staph.jpg


Positive control: K12 Negative control: Staphylococcus epidermis


Side-by-side comparison:

Motility media controls.jpg