Team:University of Lethbridge/Notebook/Project1September

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Labeled tubes and placed in teaching lab beside LB culture tubes - DO NOT MIX THESE UP!!!!
Labeled tubes and placed in teaching lab beside LB culture tubes - DO NOT MIX THESE UP!!!!
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===September 12, 2008===
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====Selina====
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Counted colonies from previous sucessful competency/transformation attempt (Aug. 25/08).
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- CaCl2 treated DH5a + pUC19 = ~3 colonies/uL (~300 colonies in 100 uL)
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- RP1616 + pUC19 = ~2 colonies/uL (~ 200 colonies in 100 uL)
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- CaCl2 treated DH5a + pTopp = ~1.8 colonies/uL (~450 colonies in 250 uL)
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- RP1616 + pTopp = ~0.2 colonies/uL (~ 20 colonies in 100 uL)

Revision as of 15:27, 12 September 2008

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Contents

September 10, 2008

Selina, Christa, Munima

Created 10 mL sterile 100 mM theophylline solution. Aliquotted into microfuge tubes and placed in cabinet in teaching lab.

Note: higher solubility in EtOH (see Merck index) but will only reach 100 mM in a super-saturated H20 solution (heat up to 50C directly before use).


September 11, 2008

Christa, Selina, Munima

Made motility media tubes (~20 blank, ~12 with 1 mM theophylline, ~10 with 0.25 mM theophylline, 1 with 50 mM theophylline). Attempts to make 'layered' motility media stab tubes was a complete disaster.

Labeled tubes and placed in teaching lab beside LB culture tubes - DO NOT MIX THESE UP!!!!


September 12, 2008

Selina

Counted colonies from previous sucessful competency/transformation attempt (Aug. 25/08).

- CaCl2 treated DH5a + pUC19 = ~3 colonies/uL (~300 colonies in 100 uL)
- RP1616 + pUC19 = ~2 colonies/uL (~ 200 colonies in 100 uL)
- CaCl2 treated DH5a + pTopp = ~1.8 colonies/uL (~450 colonies in 250 uL)
- RP1616 + pTopp = ~0.2 colonies/uL (~ 20 colonies in 100 uL)